Transcription/translation — Flashcards

A geneticist is performing an experiment to alter proteins structure. -She finds that aminoacyl tRNA synthetase in the fibroblasts loads lysine residues onto tRNA molecules containing the anticodon UUU. -This residue most likely attaches to tRNA at which site in the image below?

In an experiment, radiolabeled cystein residues are attached to their appropriate tRNAs by enzyme aminoacyt-tRNA synthetase. -The bound cystein residues are then chemically modified to form alanine. -The end product of this reaction is a tRNA moleucle that contains the cysteine anticodon but is mischarged with alanine. -What is the most likely to occur to this alanine residue during polypeptide synthesis of alpha-hemoglobin?

A researcher is studying expression pattern of aparticular gene. -Messenger RNA is isolated from several tissues, subjected to gene electrophoresis containing sequences from exon 4 of that gene. -An X-ray film is placed over the blotting membrane, with results of autoradiogram shown below: What is the best explaination to the autoradiogram findings in the different tissues?

A new experiment is being conducted to study Burkitt lymphoma. -Western blot analysis of equivalent numbers of treated and untreated cells is shown below. -What processes was most likely directly interrupted by treatment?

32 y/o man is recovering from extensive burns. -Fibrobals near site of injury actively synthesize precursor mRNA. -The finalized mRNA then exits the nucleus and undergoes translation. -Which steps involving processing and handling of mRNA occurs only within the cytoplasm of cells?

A series of experiments is being conducted to determine the structure and function of different types of bacterial RNA. -A specific RNA consisting of 90 nucleotides is purified for further analysis. -It contains high amounts of chemically modified bases such as dihydrouridine, pseudouridine, & ribothymidine, & its secondary structure aries from bases pairing within the chain. -What is the most likely composition of the 3' end of this molecules?

Describe the following points in the  tRNA?

8 y/o boy present with reduced sensitivity to pain, impaired tear formation. -Disorder is caused by loss of function of the IKAP protein. -IKAP gene sequencing reveals a single nucleotide subtitution that causes a guanine residue to be replaced by adenine. -His mRNA contains both exon and introns -What is the most likely effect of this mutation?

What are the followings in relation to processin of mRNA that only occurs within the cytoplasm of cells? -5' terminal guanosine triphosphate addition -Mehylation of the 5'terminal guanine -Multiple adenine nucleotide attachment to the 3'end -Interaction with snRNP -Removal of intervening sequences -Interaction with P bodies

NF-kB activation pathway: Pattern-Recognition Receptors recognize PAMPs which leads to activation of _____ kinase