RNA splicing and alternative splicing — Flashcards

Flashcard 1: Does prokaryotic mRNA have introns and exons?_____

Answer: No

Flashcard 2: Capped, tailed, and spliced hnRNA is called _____

Answer: mRNA

Flashcard 3: mRNA directs ribosomal synthesis of _____, which contains a signal peptide, the A and B chains of insulin, and a connecting peptide (insulin synthesis)

Answer: preproinsulin

Flashcard 4: Familial dysautonomia can be caused by a mutation at _____ sites for the IKAP gene

Answer: splice

Flashcard 5: Where does protein synthesis take place?

Answer: Ribosomes (free ribosomes in the cytoplasm or bound to the RER)

Extra: - Free ribosomes: Synthesize proteins that remain within the cytosol, or are sent to the nucleus (e.g., transcription factors), mitochondria, or peroxisomes. - RER-bound ribosomes: Synthesize proteins destined for secretion, the cell membrane, or lysosomes.

Flashcard 6: ribosome

Answer: protein synthesis

Extra: 50S and 30S subunits

Flashcard 7: Northern

Answer: RNA

Extra: complementary labeled RNA

Flashcard 8: What occurs during the translation step of collagen synthesis?

Answer: translation of preprocollagen α-chains

Flashcard 9: What kinds of proteins are synthesized by membrane bound ribosomes vs. free ribosomes?

Answer: membrane-bound - secretory proteins free - cytosolic and organellar

Flashcard 10: What functions are performed by the rough endoplasmic reticulum (RER)?

Answer: synthesis of secretory proteins and N-linked oligosaccharide