Promoters and transcription factors — Flashcards

Flashcard 1: A Northern blot is useful for studying gene _____

Answer: expression

Flashcard 2: Are enhancers/silencers close or far from the gene it regulates?_____

Answer: May be close to, far from, or within the gene (in an intron)

Flashcard 3: Zinc is a mineral that is important in the formation of _____ (transcription factor motif)

Answer: zinc fingers

Flashcard 4: While DNA polymerase binds to a primer, RNA polymerase binds to a _____

Answer: promoter

Flashcard 5: Enhancers serve as binding sites for _____ transcription factors (specific/general)

Answer: specific

Flashcard 6: Promoters serve as binding sites for _____ transcription factors (general/specific)

Answer: general

Flashcard 7: _____ are stretches of DNA that increase gene expression by binding specific transcription factors

Answer: Enhancers

Flashcard 8: Within an enhancer, there are domains known as _____, to which specific transcription factors bind

Answer: response elements (RE)

Flashcard 9: Where does protein synthesis take place?

Answer: Ribosomes (free ribosomes in the cytoplasm or bound to the RER)

Extra: - Free ribosomes: Synthesize proteins that remain within the cytosol, or are sent to the nucleus (e.g., transcription factors), mitochondria, or peroxisomes. - RER-bound ribosomes: Synthesize proteins destined for secretion, the cell membrane, or lysosomes.

Flashcard 10: ribosome

Answer: protein synthesis

Extra: 50S and 30S subunits