Transcription/translation — Flashcards

Flashcard 1: Where does protein synthesis take place?

Answer: RER

Flashcard 2: ribosome

Answer: protein synthesis

Extra: 50S and 30S subunits

Flashcard 3: Northern

Answer: RNA

Extra: complementary labeled RNA

Flashcard 4: What occurs during the translation step of collagen synthesis?

Answer: translation of preprocollagen α-chains

Flashcard 5: What kinds of proteins are synthesized by membrane bound ribosomes vs. free ribosomes?

Answer: membrane-bound - secretory proteins free - cytosolic and organellar

Flashcard 6: What functions are performed by the rough endoplasmic reticulum (RER)?

Answer: synthesis of secretory proteins and N-linked oligosaccharide

Flashcard 7: Give five covalent alterations made to proteins as post-translational modifications.

Answer: phosphorylation, glycosylation, hydroxylation, methylation, acetylation

Flashcard 8: What is the function of the E site on a ribosome?

Answer: holds empty tRNA as it exits

Flashcard 9: What is the function of the P site on a ribosome?

Answer: holds peptidyl-tRNA (tRNA holding nascent polypeptide)

Flashcard 10: What is the function of the A site on a ribosome?

Answer: holds charged tRNA