Nucleic Acid Biochemistry Indian Medical PG Flashcards - Medical Study Cards
Master Nucleic Acid Biochemistry with OnCourse flashcards. These spaced repetition flashcards are designed for medical students preparing for NEET PG, USMLE Step 1, USMLE Step 2, MBBS exams, and other medical licensing examinations.
Nucleic Acid Biochemistry Flashcard Deck - 103 Cards
Flashcard 1: Guanosine is converted to _____ via the enzyme purine nucleoside phosphorylase (purine salvage)
Answer: guanine
Flashcard 2: Hypoxanthine is converted to _____ via xanthine oxidase
Answer: xanthine
Flashcard 3: One co-transcriptional modification is the addition of a _____ at the 5' end
Answer: 7-methylguanosine cap
Flashcard 4: Inosine is converted to _____ via the enzyme purine nucleoside phosphorylase (purine salvage)
Answer: hypoxanthine
Flashcard 5: The _____ is the site where RNA polymerase II and general transcription factors bind to DNA upstream from a gene locus
Answer: promoter
Flashcard 6: microRNA often leads to the _____ of target mRNA, thus causing decreased translation into protein
Answer: silencing/inactivation
Flashcard 7: The enzymes that repair pyrimidine dimer mutations are _____ and DNA ligase
Answer: DNA polymerase
Flashcard 8: Active tetrahydrofolate is used in the synthesis of _____ and thymidine, which may then be used for DNA and RNA cell division
Answer: purines
Flashcard 9: RNA polymerase _____ makes mRNA
Answer: II
Flashcard 10: _____, the catalyst for the first reaction unique to pyrimidine biosynthesis, is a target of feedback regulation by two nucleotide triphosphates: ATP and CTP
Answer: Aspartate Transcarbamoylase
Flashcard 11: The initial mRNA of eukaryotes contains _____ and exons, which undergo splicing
Answer: introns
Flashcard 12: In base excision repair, base-specific _____ remove the altered base and create an AP (apurinic/apyrimidinic) site
Answer: glycosylases
Flashcard 13: Xanthine is converted to _____ by xanthine oxidase
Answer: uric acid
Flashcard 14: The enzymes that repair mismatched base mutations (eg. spontaneous/toxic deaminations) are _____ and DNA ligase
Answer: DNA polymerase
Flashcard 15: RNA _____ uses dsRNA complementary to an mRNA of interest to "knock down" gene expression
Answer: interference
Flashcard 16: Carbamoyl phosphate is combined with _____ to form orotic acid (pyrimidine synthesis)
Answer: aspartate
Flashcard 17: Which base excision repair enzymes are responsible for filling and sealing the gap from the excised bases?_____ (filling) and DNA ligase III (sealing)
Answer: DNA polymerase-
Flashcard 18: Xanthine may be converted to _____ via the enzyme xanthine oxidase
Answer: uric acid
Flashcard 19: When the _____ domain is removed from DNA polymerase I, the remaining fragment is called as Klenow fragment.
Answer: 5' 3' exonuclease
Flashcard 20: Ribose-5-phosphate is converted to _____ via the enzyme PRPP synthetase
Answer: phosphoribosyl pyrophosphate (PRPP)
Flashcard 21: Conversion of dUMP to dTMP via Thymidylate synthase converts the cofactor _____ to DHF
Answer: methylene THF
Flashcard 22: Every intron has a 5' _____ site and a 3' acceptor site, which are brought together to form the Lariat-shaped loop
Answer: donor
Flashcard 23: In the first step of alternative splicing, the primary transcript (hnRNA) combines with _____ and other proteins to form the spliceosome
Answer: small nuclear ribonucleoproteins (snRNPS)
Flashcard 24: In the first step of pyrimidine synthesis, glutamine and CO2 are combined to form _____ via the enzyme carbamoyl phosphate synthetase II (requires 2 ATP)
Answer: carbamoyl phosphate
Flashcard 25: Hypoxanthine may be converted to _____ via the enzyme xanthine oxidase and guanine is converted to xanthine by guanine deaminase
Answer: xanthine
Flashcard 26: Orotic acid is combined with _____ to form UMP (pyrimidine synthesis)
Answer: phosphoribosyl pyrophoshate (PRPP)
Flashcard 27: In the RISC complex, miRNAs can modulate mRNA function by stimulating _____ degradation or inhibiting translation by targeting eIF4 translation factor, in case of imperfect complimentarity
Answer: poly(A) tail
Flashcard 28: RNA polymerase _____ makes tRNA and 5S rRNA
Answer: III
Flashcard 29: _____ DNA is generated by the hydrogen bonding of a third strand into a major groove of B-DNA.
Answer: Triple strand
Flashcard 30: At the _____' end of a tRNA molecule is a 5'-CCA-3' sequence, which is the amino acid acceptor stem
Answer: 3
Flashcard 31: Primary miRNA is converted to _____ intranuclearly by the enzyme complex DROSHA
Answer: pre-miRNA
Flashcard 32: The _____ operon is a repressible operon that is turned off whenever tryptophan is added to the growth medium.
Answer: tryptophan
Flashcard 33: All 4 types of DNA are _____ handed except Z-DNA
Answer: right
Flashcard 34: The enzyme _____ is an important enzyme for de novo purine production, which converts PRPP to an intermediate used in the generation of IMP
Answer: PRPP amidotransferase
Flashcard 35: DNA & RNA are made of _____ connected via a phosphodiester bond
Answer: nucleotide monophosphates
Flashcard 36: Diphtheria toxin causes _____ of EF-2 (elongation factor-2), thus inhibiting protein synthesis
Answer: ADP-ribosylation
Flashcard 37: Cells generate purines for cell growth through either _____ production or through the salvage pathway
Answer: de novo
Flashcard 38: The _____ bond of DNA is the target of 3' hydroxyl attack (strand elongation)
Answer: triphosphate
Flashcard 39: _____ radiation results in the formation of double-stranded breaks in DNA and hydroxyl free radicals which damage DNA
Answer: Ionizing
Flashcard 40: _____ inhibits ribonucleotide reductase, which normally catalyzes conversion of UDP to dUDP (pyrimidine synthesis)
Answer: Hydroxyurea
Flashcard 41: Adenosine is converted to _____ via the enzyme adenosine deaminase (purine salvage)
Answer: inosine
Flashcard 42: Vitamins _____ and B12 are necessary for synthesis of DNA precursors
Answer: B9 (folate)
Flashcard 43: NRTIs inhibit phosphodiester bond formation in a _____ to 5' direction
Answer: 3'
Flashcard 44: _____ are small, noncoding RNA molecules that post-transcriptionally regulate protein expressiontheir 3' UTR contains sequences determining their specificity
Answer: microRNAs
Flashcard 45: A _____ mutation occurs when there is a deletion or insertion of a number of nucleotides not divisible by 3, resulting in misreading of all nucleotides downstream.
Answer: frameshift
Flashcard 46: dUMP is converted to _____ via the enzyme thymidylate synthase (with methylene THF as a cofactor)
Answer: dTMP
Flashcard 47: Pyrimidine synthesis begins with _____, CO2, and 2 ATP
Answer: glutamine
Flashcard 48: _____ of cytosine makes uracil
Answer: Deamination
Flashcard 49: Carbamoyl phosphate synthetase II is regulated by positive feedback via _____ and PRPP
Answer: ATP
Flashcard 50: Besides inhibiting Thymidine production, THF is also involved in the formation of _____, as well as some amino acids
Answer: purine nucleosides
Flashcard 51: A _____ is negatively charged DNA wrapped around the outside of positively charged proteins called histones
Answer: nucleosome (10 nm chromatin)
Flashcard 52: The enzyme which adds _____ to 3' ends of chromosomes to avoid loss of genetic material with every duplication is known as Telomerase
Answer: TTAGGG
Flashcard 53: Recognition and facilitation of excision of _____ occur via enzymes found on two genes: MSH2 (MutS) or MLH1 (MutL)
Answer: mismatched nucleotides
Flashcard 54: In response to DNA damage, p53 initially attempts to _____ the cell cycle and facilitates the activity of DNA repair enzymes*bonus-what does it induce that inhibits the cell cycle?
Answer: halt
Flashcard 55: RNA polymerase _____ makes rRNA
Answer: I
Flashcard 56: _____ is found in telomeres with parallel four-stranded DNA and has high guanine content.
Answer: G-quartet DNA
Flashcard 57: _____ polymerase must see a RNA primer to bind, which is complementary and antiparallel to the polymerase
Answer: DNA
Flashcard 58: Glutamine-PRPP amidotransferase (purine synthesis) is regulated by negative feedback via _____, IMP, and GMP
Answer: AMP
Flashcard 59: UDP is converted to _____ via the enzyme ribonucleotide reductase (pyrimidine synthesis)
Answer: dUDP
Flashcard 60: Misincorporation of uracil into DNA is seen in the deficiency of _____
Answer: folate
Flashcard 61: In _____, cuts are made several nucleotides away on either side of the damaged base.
Answer: nucleotide excision (mechanism of DNA repair)
Flashcard 62: The only mitochondrial step in pyrimidine synthesis is catalyzed by the enzyme _____
Answer: Dihydroorotate dehydrogenase
Flashcard 63: Which codon codes for tryptophan in mitochondria?_____
Answer: UGA
Flashcard 64: _____ histone dimers stabilize the primary particle and firmly bind two additional half-turns of DNA
Answer: H2AH2B
Flashcard 65: What is the direction of DNA repair?_____
Answer: 5' to 3'
Flashcard 66: Non-canonical DNA and RNA segments are formed as a result of _____
Answer: secondary structures
Flashcard 67: Which nitrogenous base contains an amino group on the 4th C-atom?_____
Answer: Cytosine
Flashcard 68: Denatured DNA shows hyperchromicity at _____nm
Answer: 260
Flashcard 69: Where does RNA splicing occur inside the cell?_____
Answer: Nucleus
Flashcard 70: Restriction endonucleases are present only in cells that also have a companion enzyme that site-specifically _____ the host DNA
Answer: methylates
Flashcard 71: In all cells, replication can occur only from a _____-stranded DNA template
Answer: single (double/single)
Flashcard 72: Which nitrogenous base contains an amino group on the 6th C-atom?_____
Answer: Adenine
Flashcard 73: Which codons serve as chain terminators in mitochondria?_____
Answer: AGA and AGG
Flashcard 74: The miRNA gene present in the nucleus is transcribed to _____ miRNA
Answer: primary
Flashcard 75: Leading strand synthesis is carried out by DNA polymerase _____.
Answer:
Flashcard 76: Which enzyme is an alternative pathway for conversion of Adenine to AMP via the salvage pathway?_____
Answer: Adenine phosphoribosyltransferase (not HGPRT)
Flashcard 77: What products are formed by the catabolism of cytosine and uracil?_____
Answer: -alanine, CO2 and NH3.
Flashcard 78: Which eIF binds to the 5' terminal of mRNA molecles which are capped?_____
Answer: eIF4
Flashcard 79: Unwinding /Denaturation of DNA is started by _____
Answer: Ori-binding protein.
Flashcard 80: Which nitrogenous base contains a methyl group on the 5th C-atom?_____
Answer: Thymine
Flashcard 81: H2A, H2B, H3 and H4 are called _____ histones.
Answer: core
Flashcard 82: Viscosity of DNA _____ when it undergoes denaturation.
Answer: decreases
Flashcard 83: RNA editing occurs in _____ direction
Answer: 3 to 5
Flashcard 84: Okazaki fragments are lagging strands built on RNA primers by DNA polymerase _____.
Answer:
Flashcard 85: In Sanger sequencing, dideoxynucleotides triphosphates are used because it doesn't allow further _____ of DNA.
Answer: elongation
Flashcard 86: What kind of DNA is found in conditions of high humidity and low salt concentration?_____
Answer: B-DNA
Flashcard 87: _____ bases cannot be salvaged to their corresponding nucleotides.
Answer: Pyrimidine
Flashcard 88: Is the primary DNA structure altered when it undergoes denaturation?_____
Answer: No
Flashcard 89: Which enzyme gives signals for termination of RNA transcription?_____
Answer: RNA polymerase II
Flashcard 90: Which RNA subtypes do not require capping at 5' end?_____
Answer: tRNA and rRNA
Flashcard 91: DNA repair is carried out by DNA polymerase _____.
Answer:
Flashcard 92: Mitochondrial DNA has a _____ mutation rate than that of nuclear DNA
Answer: higher (higher/lower)
Flashcard 93: What is the direction of RNA editing?_____
Answer: 3 to 5
Flashcard 94: Which nitrogenous base contains an amino group on the 2nd C-atom?_____
Answer: Guanine
Flashcard 95: What products are formed by the catabolism of thymine?_____
Answer: -aminoisobutyrate, CO2 and NH3.
Flashcard 96: Human mitochondria contain 2 to 10 copies of a small circular DNA that makes up approximately _____% of total cellular DNA.
Answer: 1
Flashcard 97: Polyadenylation of mRNA protects against degradation by _____
Answer: 3 exonucleases
Flashcard 98: Which eIF is involved in the formation of binary complex (GTP+eIF)?_____
Answer: eIF2
Flashcard 99: What kind of DNA is found in conditions of low humidity and high salt concentration?_____
Answer: A-DNA
Flashcard 100: RNA primer synthesis is carried out by DNA polymerase _____.
Answer:
Flashcard 101: The 3 end with the CCA sequence is the _____ arm of the tRNA
Answer: acceptor
Flashcard 102: The disadvantage of using CDPA-1 is that it increases _____ stone formation.
Answer: uric acid
Flashcard 103: _____ deficiency causes Orotic aciduria
Answer: UMP synthase
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