NEET-PG 2015

1414 Questions — Page 59 of 142

Biochemistry

2 questions

Q581

Which of the following acids is MOST commonly used as a reagent in medical laboratory analytical procedures?

Q582

Shadow casting is used in -

NEET-PG 2015 - Biochemistry NEET-PG Practice Questions and MCQs

Question 581: Which of the following acids is MOST commonly used as a reagent in medical laboratory analytical procedures?

A. Nitric acid is used in chemical analysis.
B. Carbolic acid is used as a disinfectant.
C. Oxalic acid is used in various laboratory applications.
D. Sulphuric acid is used in various laboratory processes. (Correct Answer)

Explanation: ***Sulphuric acid*** is the most commonly used acid in medical laboratory analytical procedures. - **Sulfuric acid (H₂SO₄)** is a strong mineral acid with the **widest range of applications** in clinical and research laboratories - Used extensively as a **catalyst and reagent** in numerous analytical procedures including **protein digestion**, **Kjeldahl nitrogen estimation**, and **enzymatic assays** - Essential in **sample preparation** for heavy metal analysis and trace element detection - Utilized in **deproteinization** procedures and various **colorimetric assays** - Its strong **dehydrating properties** make it valuable in multiple biochemical protocols *Nitric acid* - **Nitric acid (HNO₃)** is primarily used for **acid digestion** of samples in trace element analysis - Strong **oxidizing agent** but has more **specialized applications** compared to sulfuric acid - More commonly used in **environmental and toxicology testing** than routine clinical biochemistry - Its highly **corrosive and oxidizing nature** limits its use in routine procedures *Carbolic acid* - **Carbolic acid (phenol/C₆H₅OH)** is technically not a mineral acid but a weak organic acid - Historically used as an **antiseptic and disinfectant** (Lister's antiseptic) - Modern laboratory use is **limited** to specific applications like **phenol-chloroform extraction** in molecular biology - Due to **toxicity concerns**, largely replaced by safer alternatives in routine disinfection *Oxalic acid* - **Oxalic acid (C₂H₂O₄)** is an organic dicarboxylic acid with **specialized applications** - Used in **decalcification of bone samples** for histopathology - Functions as a **reducing agent** in specific analytical procedures - Not a routine reagent in general medical laboratory practice compared to sulfuric acid

Question 582: Shadow casting is used in -

A. Light microscopy
B. Electron microscopy (Correct Answer)
C. Fluorescence microscopy
D. Phase contrast microscopy

Explanation: ***Electron microscopy*** - **Shadow casting** is a technique used in **electron microscopy** to enhance contrast and reveal the three-dimensional topography of small structures and molecules by depositing a thin film of heavy metal at an angle. - This process creates areas with more metal (which appears darker) and areas shielded from the metal deposition (appearing lighter, like a shadow), thereby outlining the specimen. *Light microscopy* - **Light microscopy** uses visible light to illuminate specimens and a system of lenses to magnify images, and it does not typically employ shadow casting techniques for contrast enhancement. - While various techniques like staining are used for contrast, the principle of creating shadows by metal deposition is not applicable to light interactions with the sample. *Fluorescence microscopy* - **Fluorescence microscopy** utilizes the property of some substances to emit light of a longer wavelength when excited by light of a shorter wavelength (fluorescence), and it relies on fluorochromes for visualization, not shadow casting. - This technique creates contrast based on specific labels or autofluorescence, highlighting particular structures without direct shadowing. *Phase contrast microscopy* - **Phase contrast microscopy** converts phase shifts in light passing through a transparent specimen into changes in amplitude (brightness), which are then visible as differences in image contrast, and it does not involve metal deposition or shadow casting. - This method is particularly useful for observing live, unstained biological samples by detecting optical path differences.

Forensic Medicine

3 questions

Q581

Which vein is most commonly used for blood collection during post-mortem examinations?

Q582

Which form of lead is considered the least toxic?

Q583

What is the scientific name of the plant commonly known as Thorn apple?

NEET-PG 2015 - Forensic Medicine NEET-PG Practice Questions and MCQs

Question 581: Which vein is most commonly used for blood collection during post-mortem examinations?

A. Femoral vein (Correct Answer)
B. Antecubital vein
C. Jugular vein
D. Carotid artery

Explanation: ***Femoral vein*** - The **femoral vein** in the groin region is frequently chosen for post-mortem blood collection because it is large, superficial, and easily accessible, even in cases of significant trauma or decomposition. - Blood from the femoral vein is generally considered less prone to **post-mortem redistribution** of drugs and endogenous substances compared to central veins. *Antecubital vein* - The **antecubital vein** (in the elbow crease) is a common site for blood collection in living individuals but is less preferred post-mortem due to its smaller size and potential for damage or disfigurement during autopsy. - Its accessibility can be limited depending on the body's condition and positioning during a post-mortem examination. *Jugular vein* - While large, the **jugular vein** in the neck is generally less preferred for routine post-mortem blood collection than the femoral vein due to its proximity to the heart and great vessels. - Blood samples from the jugular vein may be more significantly affected by **post-mortem redistribution** of substances due to fluid shifts. *Carotid artery* - The **carotid artery** is an artery, not a vein, and is typically avoided for routine post-mortem blood collection unless specific arterial samples are required. - Accessing an artery can be more challenging and may provide blood that is less representative of general systemic circulation compared to venous samples for toxicology.

Question 582: Which form of lead is considered the least toxic?

A. Lead acetate
B. Lead oxide
C. Lead carbonate
D. Lead sulphide (Correct Answer)

Explanation: **Correct Option: Lead sulphide** - **Lead sulphide (galena)** has very low solubility in biological systems, meaning it is poorly absorbed by the body. - Due to its poor absorption and insolubility, it is considered the **least toxic** form of lead among the options listed. - It is essentially inert in the gastrointestinal tract and passes through without significant absorption. *Incorrect: Lead acetate* - **Lead acetate** is a highly soluble lead compound and is therefore readily absorbed by the body, making it significantly toxic. - It has been historically used in various applications (sugar of lead), increasing exposure risk and demonstrating its high bioavailability. - Soluble salts of lead are the most dangerous forms. *Incorrect: Lead oxide* - **Lead oxide** (like litharge or red lead) is moderately soluble and can be absorbed through ingestion or inhalation, contributing to lead toxicity. - It is used in paints and batteries, leading to industrial and environmental exposure risks. - Chronic exposure can lead to significant lead accumulation. *Incorrect: Lead carbonate* - **Lead carbonate** (white lead) is also a relatively soluble lead compound and is readily absorbed by the body, making it highly toxic. - It was historically used extensively in paints and cosmetics, contributing to significant lead poisoning cases. - The solubility in gastric acid makes this form particularly dangerous when ingested.

Question 583: What is the scientific name of the plant commonly known as Thorn apple?

A. Datura stramonium (Correct Answer)
B. Nux vomica
C. Papaver somniferum
D. Cannabis sativa

Explanation: ***Datura stramonium*** - This is the correct **scientific name** for the plant commonly known as **Thorn apple** or Jimsonweed. - *Datura stramonium* is known for its **anticholinergic** properties due to alkaloids like atropine, scopolamine, and hyoscyamine. *Nux vomica* - This plant is scientifically known as *Strychnos nux-vomica* and is a source of **strychnine** and brucine. - It is known for its **CNS stimulant** and convulsant effects, and is not referred to as Thorn apple. *Papaver somniferum* - This is the **opium poppy**, famous for producing **opium**, from which morphine, codeine, and heroin are derived. - Its common name is not Thorn apple, and its effects are primarily **analgesic** and sedative. *Cannabis sativa* - This plant is commonly known as **marijuana** or hemp, and it produces cannabinoids such as THC and CBD. - It is recognized for its **psychoactive** and medicinal properties, and is not related to Thorn apple.

Microbiology

2 questions

Q581

Which bacteria can be isolated using crystal violet blood agar?

Q582

Which of the following statements is NOT true about the El Tor biotype of Vibrio cholerae?

NEET-PG 2015 - Microbiology NEET-PG Practice Questions and MCQs

Question 581: Which bacteria can be isolated using crystal violet blood agar?

A. Corynebacterium diphtheriae
B. Staph aureus
C. Meningococcus
D. β-hemolytic streptococci (Correct Answer)

Explanation: ***β-hemolytic streptococci*** - **Crystal violet blood agar** is a selective medium that inhibits the growth of most Gram-positive bacteria, except for **beta-hemolytic streptococci**. - The crystal violet dye suppresses the growth of competing flora, allowing for better isolation and identification of these bacteria, which exhibit **complete hemolysis (beta-hemolysis)** on blood agar. *Corynebacterium diphtheriae* - This bacterium requires more specialized media, such as **Tinsdale agar** or **Loeffler's serum agar**, for optimal growth and identification due to specific nutritional requirements and colony morphology. - Crystal violet blood agar is not the primary medium used for its isolation. *Staph aureus* - **Staphylococcus aureus** is a common contaminant that is typically inhibited by the crystal violet in the medium. - It grows well on routine blood agar but is not selectively grown or isolated using crystal violet blood agar. *Meningococcus* - **Neisseria meningitidis** (Meningococcus) requires enriched media like **chocolate agar** or **Thayer-Martin agar** for successful isolation, as it is a fastidious organism. - Crystal violet blood agar is not suitable for its growth due to its inhibitory properties and lack of necessary nutrients.

Question 582: Which of the following statements is NOT true about the El Tor biotype of Vibrio cholerae?

A. VP (+)
B. Lower mortality
C. Reduced environmental persistence (Correct Answer)
D. Hemolysis negative

Explanation: ***Reduced environmental persistence*** - The **El Tor biotype** of *Vibrio cholerae* is known for its **increased environmental persistence** compared to the classical biotype, making this statement NOT true. - El Tor survives longer in water sources due to its hardiness and ability to form biofilms, which contributes to its pandemic potential and makes outbreaks harder to control. *VP (+)* - The El Tor biotype is **Voges-Proskauer (VP) positive**, which is a key biochemical characteristic used to differentiate it from the classical biotype (VP negative). - This is a TRUE statement about El Tor. *Lower mortality* - The El Tor biotype causes **milder disease with lower mortality rates** compared to the classical biotype. - While individual cases may be less severe, the higher infectivity and asymptomatic carriage of El Tor contribute to its widespread transmission - this is a TRUE statement. *Hemolysis negative* - The El Tor biotype is **hemolysis positive** (produces beta-hemolysis on sheep blood agar), which is another key differentiating feature from the classical biotype (hemolysis negative). - This makes the statement "hemolysis negative" NOT true about El Tor.

Pathology

1 questions

Q581

Which tumor is characterized by the presence of cells that resemble tennis rackets?

Pharmacology

2 questions

Q581

In the context of pharmacology, what is the term 'Mickey Finn' commonly associated with?

Q582

From which part of the Papaver somniferum plant does the latex, commonly referred to as 'milk', exude?

NEET-PG 2015 - Pharmacology NEET-PG Practice Questions and MCQs

Question 581: In the context of pharmacology, what is the term 'Mickey Finn' commonly associated with?

A. Chloroform
B. Methyl alcohol
C. Chloral hydrate (Correct Answer)
D. Ethylene glycol

Explanation: ***Chloral hydrate*** - A "Mickey Finn" is a slang term for a drink **laced with a psychoactive drug or incapacitating agent** given to an unsuspecting person. - Historically, **chloral hydrate** was a common substance used for this purpose due to its rapid sedative-hypnotic effects. *Chloroform* - While chloroform is a potent anesthetic and sedative, it is primarily used as an **inhalant** and is not typically administered orally in drinks. - Ingesting chloroform in large quantities can be **fatal due to severe hepatotoxicity and neurotoxicity**. *Methyl alcohol* - **Methyl alcohol (methanol)** is highly toxic and causes severe metabolic acidosis, blindness, and death, even in small amounts. - It does not induce the quick, incapacitating sedative effects associated with a "Mickey Finn" but rather a **delayed, severe poisoning syndrome**. *Ethylene glycol* - **Ethylene glycol** is an antifreeze agent that is also highly toxic, causing kidney failure and metabolic derangements. - Similar to methanol, its effects are **delayed and severe**, not the immediate incapacitating sedation implied by the term "Mickey Finn."

Question 582: From which part of the Papaver somniferum plant does the latex, commonly referred to as 'milk', exude?

A. Leaf of the plant
B. Root of the plant
C. Seeds of the plant
D. Unripe capsule of the plant (Correct Answer)

Explanation: ***Unripe capsule of the plant*** - The **latex** (or 'milk') containing **opioid alkaloids** like morphine and codeine is primarily harvested by incising the **unripe seed capsules** of the *Papaver somniferum* plant. - This milky sap is then collected and dried to produce **crude opium**. *Leaf of the plant* - The leaves of *Papaver somniferum* do not contain significant amounts of the latex and are not the primary source of **opium alkaloids**. - While some **alkaloids** might be present in trace amounts, they are not extracted commercially from the leaves. *Root of the plant* - The roots of the poppy plant are not known to exude latex or to be a significant source of medically relevant **opioid alkaloids**. - Their primary function is absorption of water and nutrients, and anchoring the plant. *Seeds of the plant* - While the dried seeds are used for culinary purposes (poppy seeds), they contain very low levels of **opioid alkaloids** compared to the latex. - The latex is produced within the **capsule** before the seeds fully mature.