INI-CET 2018 - Microbiology PYQs Practice Questions

Q: Post kidney transplantation, a patient presents with diarrhoea. The motility of the worms is shown in the figure. Correct statement about the organism is: (AIIMS Nov 2018)

Loeffler pneumonia is caused by the same organism. ***Loeffler pneumonia is caused by the same organism*** - The image shows **Strongyloides stercoralis larvae** with characteristic motility. In a post-kidney transplant patient with diarrhea, this points to **Strongyloides hyperinfection** due to immunosuppression. - **Loeffler's syndrome (Loeffler pneumonia)** is a transient pulmonary infiltrative disease with eosinophilia, caused by larval migration through lungs. It is classically associated with *Strongyloides stercoralis*, *Ascaris lumbricoides*, and hookworms during their migratory phase. - This option represents an important **clinical association** that connects the organism to a significant pulmonary manifestation. *Monoecious organism related with parthenogenesis* - *Strongyloides stercoralis* is **dioecious (unisexual)**, not monoecious, meaning it has separate male and female forms. - While the parasitic females do exhibit **parthenogenesis** (reproducing without fertilization), the organism is not monoecious. *Transmitted by intake of contaminated food and water* - This is **incorrect**. *Strongyloides stercoralis* is transmitted through **percutaneous penetration** by filariform larvae from contaminated soil. - Fecal-oral transmission via contaminated food/water applies to parasites like *Entamoeba histolytica*, *Giardia*, *Ascaris*, and *Enterobius*, but not *Strongyloides*. *Infection occurs by filariform larvae* - This statement is **factually correct** - infection with *Strongyloides* does occur through **filariform (L3) larvae** penetrating intact skin. - However, in the context of this PYQ, Option C is preferred as it highlights the **clinically significant pulmonary manifestation** (Loeffler's syndrome) that is particularly relevant in immunocompromised patients, while this option merely describes the mode of transmission without clinical correlation. [Practice more Microbiology PYQs on OnCourse]

Q: The technique shown in the picture is used for: (AIIMS Nov 2018)

Synthesis of monoclonal antibodies. ***Synthesis of monoclonal antibodies*** - The image depicts the process of creating **hybridomas** by fusing antibody-producing spleen cells from an immunized mouse with myeloma cells. - These hybridomas are then cultured in **HAT medium** to select for stable cell lines that continuously produce a single type of antibody, known as **monoclonal antibodies**. *Preparing cell lines for viral culture* - While cell cultures are used for viral studies, the specific methodology shown involving immunization, spleen cell extraction, and fusion with myeloma cells is not for preparing routine **viral cultures**. - Viral culture typically involves growing target cells that are susceptible to a virus and then inoculating them with the virus for replication and study. *Synthesis of vaccine* - **Vaccine synthesis** involves producing antigens (e.g., inactivated viruses, attenuated microbes, or recombinant proteins) to stimulate an immune response, not the production of antibodies themselves. - The process shown aims to generate antibodies, which could *potentially* be used therapeutically, but not primarily for vaccine production. *Process of genetic engineering* - **Genetic engineering** involves manipulating an organism's genes, such as introducing foreign DNA or modifying existing genes. - While molecular biology techniques are involved in antibody production, the core process of fusing cells to create hybridomas specifically for large-scale antibody production is distinct from typical genetic engineering methods like gene cloning or gene editing. [Practice more Microbiology PYQs on OnCourse]

Question 1

Post kidney transplantation, a patient presents with diarrhoea. The motility of the worms is shown in the figure. Correct statement about the organism is: (AIIMS Nov 2018)

Accepted Answer

Loeffler pneumonia is caused by the same organism

Answer

Monoecious organism related with parthenogenesis

Answer

Transmitted by intake of contaminated food and water

Answer

Infection occurs by filariform larvae

Question 2

The technique shown in the picture is used for: (AIIMS Nov 2018)

Accepted Answer

Synthesis of monoclonal antibodies

Answer

Preparing cell lines for viral culture

Answer

Synthesis of vaccine

Answer

Process of genetic engineering

INI-CET 2018 — Microbiology