Glycolysis — Flashcards

Flashcard 11: What are the kinetic properties of glucokinase?

Answer: low affinity, high capacity

Flashcard 12: What are the kinetic properties of hexokinase?

Answer: high affinity, low capacity

Flashcard 13: How many ATP are produced by anerobic glycolysis?

Answer: 2 ATP/glucose (net)

Flashcard 14: What is the rate-limiting enzyme of glycolysis?

Answer: Phosphofructokinase-1 (PFK-1)

Extra: Regulation of PFK-1: - Activators: AMP, Fructose-2,6-bisphosphate - Inhibitors: ATP, Citrate

Flashcard 15: Glucose is converted to glucose-6-phosphate by the enzyme _____ in most tissues

Answer: hexokinase

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Flashcard 16: In erythrocytes, 90% of glucose is used for _____

Answer: glycolysis

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Flashcard 17: Which glycolytic enzyme deficiency is the **second** most common cause of enzyme-deficient hemolytic anemia? _____

Answer: Pyruvate kinase deficiency

Extra: * G6PD deficiency is the most common cause of enzyme-deficient hemolytic anemia (it is an HMP shunt enzyme). * Pyruvate kinase deficiency is the most common glycolytic enzyme deficiency.

Flashcard 18: Phosphofructokinase-1 is activated (positive allosteric regulation) by _____ and fructose-2,6-bisphosphate

Answer: AMP

Extra: PFK-1 is the rate-limiting enzyme of glycolysis. - **Allosteric Activators:** AMP, Fructose-2,6-bisphosphate (indicates low energy/fed state) - **Allosteric Inhibitors:** ATP, Citrate (indicates high energy state)

Flashcard 19: 1,3-bisphosphoglycerate is converted into _____ via the enzyme phosphoglycerate kinase

Answer: 3-phosphoglycerate

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Flashcard 20: Conversion of pyruvate -> lactate (Cori cycle) recycles one molecule of _____

Answer: NAD+

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