Preparatory phase reactions US Medical PG Flashcards - Medical Study Cards
Master Preparatory phase reactions with OnCourse flashcards. These spaced repetition flashcards are designed for medical students preparing for NEET PG, USMLE Step 1, USMLE Step 2, MBBS exams, and other medical licensing examinations.
Preparatory phase reactions Flashcard Deck - 10 Cards
Flashcard 1: Hexokinase is regulated via negative feedback by _____
Answer: glucose-6-phosphate
Flashcard 2: Which two steps of glycolysis produce ATP?
Answer: 1. 1,3-bisphosphoglycerate → 3-phosphoglycerate (catalyzed by phosphoglycerate kinase)
2. Phosphoenolpyruvate → pyruvate (catalyzed by pyruvate kinase)
Extra: These steps are examples of substrate-level phosphorylation. Although 4 ATP are produced in total during the payoff phase, the net gain of glycolysis is 2 ATP (because 2 ATP are consumed in the investment phase).
Flashcard 3: Phosphofructokinase-2 (PFK-2) is activated by the hormone _____
Answer: Insulin
Extra: In the fed state, insulin leads to the dephosphorylation of the PFK-2/FBPase-2 bifunctional enzyme, which activates the PFK-2 domain. This increases Fructose-2,6-bisphosphate (F-2,6-BP) levels, which is the most potent allosteric activator of PFK-1 (the rate-limiting enzyme of glycolysis).
Flashcard 4: Anaerobic glycolysis produces a net of _____ ATP per glucose molecule
Answer: 2
Flashcard 5: In the liver, Phosphofructokinase-2 (PFK-2) is inhibited by the hormone _____
Answer: Glucagon
Flashcard 6: What is the rate-limiting enzyme of glycolysis?_____
Answer: Phosphofructokinase-1 (PFK-1)
Flashcard 7: What is the function of the bifunctional enzyme Phosphofructokinase-2/Fructose bisphosphatase-2 (PFK-2/FBP-2)?
Answer: A bifunctional enzyme that regulates the levels of Fructose-2,6-bisphosphate (F-2,6-BP), thereby balancing glycolysis and gluconeogenesis.
Extra: In the well-fed state, PFK-2 is active (increased F-2,6-BP) → stimulates Glycolysis.\nIn the fasting state, FBP-2 is active (decreased F-2,6-BP) → stimulates Gluconeogenesis.
Flashcard 8: Where does glycolysis take place?
Answer: cytoplasm
Flashcard 9: What is the rate-limiting enzyme of glycolysis?
Answer: Phosphofructokinase-1 (PFK-1)
Extra: The three irreversible steps of glycolysis are catalyzed by:\n1. Hexokinase/Glucokinase\n2. Phosphofructokinase-1 (Rate-limiting step)\n3. Pyruvate kinase\n\nPFK-1 is inhibited by ATP and Citrate, and activated by AMP and Fructose-2,6-bisphosphate.
Flashcard 10: What enzyme converts pyruvate to lactate?
Answer: lactate dehydrogenase (LDH)
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