Biochemical Techniques — Flashcards

Flashcard 11: When proteins containing _____ react with diazotized sulfanilic acid under alkaline conditions, red color is formed by a coupling reaction is the principle behind Pauly's test

Answer: histidine

Flashcard 12: Light absorbing capacity of _____ amino acids is used to estimate the protein concentration with a spectrophotometer

Answer: aromatic

Flashcard 13: In the _____ test for urobilin, zinc acetate is used to produce a characteristic green fluorescence.

Answer: Schlesinger's test

Extra: Schlesinger's test is used to detect **urobilin**. 1. If urobilinogen is present, it is first oxidized to urobilin (e.g., using iodine). 2. **Zinc acetate** is then added, which reacts with urobilin to form a compound that exhibits a characteristic **green fluorescence**.

Flashcard 14: _____ is a calcium staining technique which is used on DuPont Automatic Clinical Analyzer (ACA) to measure serum calcium photometrically.

Answer: Alizarin Red S

Flashcard 15: Compounds with _____ absorb UV light.

Answer: conjugated double bonds

Flashcard 16: In _____ test for bile pigments, concentrated nitric acid is used as an oxidizing agent

Answer: Gmelin's

Flashcard 17: Salting out of proteins from a solution can be done using _____

Answer: ammonium sulfate

Flashcard 18: Xanthoproteic reaction is used to check for _____ groups

Answer: benzene

Flashcard 19: Ninhydrin reaction is used to check for _____

Answer: alpha amino acids

Flashcard 20: _____ is the strongest organic acid present in the body.

Answer: Heparin