Spectrophotometry and Colorimetry — Flashcards

Flashcard 1: _____ gives DNA a green fluorescence and RNA a red fluorescence

Answer: Acridine orange

Flashcard 2: Light absorbing capacity of _____ amino acids is used to estimate the protein concentration with a spectrophotometer

Answer: aromatic

Flashcard 3: In the _____ test for urobilin, zinc acetate is used to produce a characteristic green fluorescence.

Answer: Schlesinger's test

Extra: Schlesinger's test is used to detect **urobilin**. 1. If urobilinogen is present, it is first oxidized to urobilin (e.g., using iodine). 2. **Zinc acetate** is then added, which reacts with urobilin to form a compound that exhibits a characteristic **green fluorescence**.

Flashcard 4: Compounds with _____ absorb UV light.

Answer: conjugated double bonds

Flashcard 5: The _____ test is the most definitive test for detecting the presence of blood.

Answer: spectroscopic

Flashcard 6: LJ charts give _____ and _____ of a test

Answer: accuracy

Extra: Levey-Jennings (LJ) charts are used in laboratory quality control to monitor both accuracy (systematic error) and precision (random error). In contrast: - **Mean charts** monitor accuracy. - **Range (R) charts** monitor precision.

Flashcard 7: membrane pore size is _____ in RO(reverse osmosis)

Answer: <0.002 micrometer

Extra: 

Flashcard 8: A _____ blot is used to analyze DNA

Answer: Southern

Flashcard 9: In the first step of PCR, DNA is _____ by using heat to separate the strands

Answer: denatured

Flashcard 10: When using ELISA, detection involves the use of an _____ linked to an enzyme

Answer: antibody