Chromatography Techniques — Flashcards

Flashcard 1: Composition of Benedict s reagent: Sodium citrate, Sodium _____ and Copper sulfate

Answer: carbonate

Flashcard 2: Which ELISA is used for hapten detection?_____

Answer: Competitive ELISA

Flashcard 3: Ninhydrin reaction is used to check for _____

Answer: alpha amino acids

Flashcard 4: In the _____ test for urobilin, zinc acetate is used to produce a characteristic green fluorescence.

Answer: Schlesinger's test

Extra: Schlesinger's test is used to detect **urobilin**. 1. If urobilinogen is present, it is first oxidized to urobilin (e.g., using iodine). 2. **Zinc acetate** is then added, which reacts with urobilin to form a compound that exhibits a characteristic **green fluorescence**.

Flashcard 5: In the first step of PCR, DNA is _____ by using heat to separate the strands

Answer: denatured

Flashcard 6: LJ charts give _____ and _____ of a test

Answer: accuracy

Extra: Levey-Jennings (LJ) charts are used in laboratory quality control to monitor both accuracy (systematic error) and precision (random error). In contrast: - **Mean charts** monitor accuracy. - **Range (R) charts** monitor precision.

Flashcard 7: membrane pore size is _____ in RO(reverse osmosis)

Answer: <0.002 micrometer

Extra: 

Flashcard 8: A _____ blot is used to analyze DNA

Answer: Southern

Flashcard 9: When using ELISA, detection involves the use of an _____ linked to an enzyme

Answer: antibody