Biochemical Techniques — Flashcards

Flashcard 1: LJ charts give _____ and _____ of a test

Answer: validity

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Flashcard 2: membrane pore size is _____ in RO(reverse osmosis)

Answer: <0.002 micrometer

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Flashcard 3: A _____ blot is used to analyze DNA

Answer: Southern

Flashcard 4: In the first step of PCR, DNA is _____ by using heat to separate the strands

Answer: denatured

Flashcard 5: When using ELISA, detection involves the use of an _____ linked to an enzyme

Answer: antibody

Flashcard 6: Lipoproteins with _____ protein content will move faster towards the anode during electrophoresis

Answer: higher

Flashcard 7: A _____ blot is used to analyze protein

Answer: Western

Flashcard 8: _____ gives DNA a green fluoresence and RNA a red fluoresence

Answer: Acridine orange

Flashcard 9: A _____ blot is used to identify DNA-binding proteins using labeled oligonucleotide probes

Answer: Southwestern

Flashcard 10: In the final step of PCR, heat-stable _____ elongates the DNA sequence following each primer

Answer: Taq DNA polymerase

Flashcard 11: Light absorbing capacity of _____ amino acids is used to estimate the protein concentration with a spectrophotometer

Answer: aromatic

Flashcard 12: When proteins containing _____ react with diazotized sulfanilic acid under alkaline conditions, red color is formed by a coupling reaction is the principle behind Pauly's test

Answer: histidine

Flashcard 13: _____ is a calcium staining technique which is used on DuPont Automatic Cyclical Analyser (ACA) to measure serum calcium photometrically.

Answer: Alizarin red S

Flashcard 14: Compounds with _____ absorb UV light.

Answer: conjugated double bonds

Flashcard 15: In _____ test for bile pigments, zinc is used as an oxidizing agent

Answer: Schlesinger's

Flashcard 16: In _____ test for bile pigments, nitric oxide is used as an oxidizing agent

Answer: Gmelin's

Flashcard 17: Salting out of proteins from a solution can be done using _____

Answer: ammonium sulfate

Flashcard 18: Xanthoproteic reaction is used to check for _____ groups

Answer: benzene

Flashcard 19: Ninhydrin reaction is used to check for _____

Answer: alpha amino acids

Flashcard 20: _____ is the strongest organic acid present in the body.

Answer: Heparin

Flashcard 21: Osazone test is a routine test that is done to identify the _____ sugars

Answer: reducing

Flashcard 22: The technique of the Osazone test was first developed by _____, a German chemist.

Answer: Emil Fischer

Flashcard 23: The technique used to detect DNA-protein interaction is DNA _____

Answer: footprinting

Flashcard 24: Which ELISA is used for hapten detection?_____

Answer: Competitive ELISA

Flashcard 25: In _____ test for bile pigments, para dimethyl amino benzaldehyde react to form red color

Answer: Ehrlich's aldehyde reagent

Flashcard 26: Sakaguchi reaction is used to check for _____ groups

Answer: guanidine

Flashcard 27: Composition of Benedict s reagent: Sodium citrate, Sodium _____ and Copper sulfate

Answer: carbonate

Flashcard 28: Which medium is used in hybridoma techinique to allow only fused cells to survive in culture?_____

Answer: HAT medium

Flashcard 29: Nitroprusside reaction is used to check for _____ groups

Answer: sulfhydryl

Flashcard 30: _____ is a nucleic acid selective fluorescent cationic dye useful for cell cycle discrimination

Answer: Acridine orange

Flashcard 31: Osazones are crystals that are formed when the sugars react with a compound known as _____, at boiling point.

Answer: phenylhydrazine

Flashcard 32: The _____ test is the most definitive test for detecting the presence of blood.

Answer: spectroscopic