Enzymes Practice Questions

Q: Which enzyme is primarily stored in muscle?

SGOT. **Explanation:** The correct answer is **SGOT (Serum Glutamic Oxaloacetic Transaminase)**, also known as **AST (Aspartate Aminotransferase)**. While many enzymes are found in muscle tissue, the question asks which is "primarily stored" or highly concentrated there. AST is found in high concentrations in the **cardiac muscle, skeletal muscle**, and liver. In the context of muscle metabolism, AST plays a crucial role in the malate-aspartate shuttle and amino acid metabolism. When muscle tissue is damaged (e.g., rhabdomyolysis or myocardial infarction), AST levels rise significantly in the serum. **Analysis of Incorrect Options:** * **A. Alkaline Phosphatase (ALP):** Primarily found in the liver (bile duct epithelium), bone (osteoblasts), placenta, and intestine. It is a marker for obstructive jaundice and bone turnover, not muscle. * **C. SGPT (ALT):** While present in muscles, ALT is considered **liver-specific**. Its concentration in the liver is much higher than in any other tissue, making it the preferred marker for hepatocellular injury. * **D. CPK (Creatine Phosphokinase):** Although CPK is highly abundant in muscle (specifically the CK-MM and CK-MB isoenzymes), the NEET-PG pattern often distinguishes between "storage" and "activity." While CPK is a more *sensitive* marker for muscle damage, AST is historically categorized in biochemistry as being stored in high quantities across both cardiac and skeletal muscle groups. **NEET-PG High-Yield Pearls:** * **De Ritis Ratio:** AST/ALT ratio > 2 is suggestive of Alcoholic Liver Disease. * **Myocardial Infarction (MI):** AST was historically used as a cardiac marker; it rises 6–8 hours after an MI, peaks at 24 hours, and returns to normal by day 5. * **Tissue Distribution of AST:** Heart > Liver > Skeletal Muscle > Kidney > RBCs.

Q: Trypsin is an activator of all of the following enzymes EXCEPT?

Pepsinogen. **Explanation:** The activation of pancreatic enzymes is a critical step in digestion, initiated by a cascade mechanism. **Trypsin** acts as the common activator for almost all proteolytic and lipolytic zymogens secreted by the pancreas. **Why Pepsinogen is the correct answer:** Pepsinogen is a gastric proenzyme secreted by the **Chief cells** of the stomach. Its activation into **Pepsin** is mediated by the acidic environment (low pH) of the stomach and by **auto-activation** (pepsin activating more pepsinogen). Trypsin, which operates in the alkaline environment of the duodenum, has no role in the activation of gastric pepsinogen. **Analysis of incorrect options:** * **Chymotrypsinogen:** Trypsin cleaves the peptide bond between Arginine-15 and Isoleucine-16 to convert it into active $\pi$-chymotrypsin. * **Proelastase:** Trypsin activates proelastase into **Elastase**, which breaks down elastin in connective tissues. * **Procolipase:** Trypsin converts procolipase into **Colipase**, which is essential for the action of pancreatic lipase in the presence of bile salts. **High-Yield NEET-PG Pearls:** 1. **The Master Switch:** **Enteropeptidase** (Enterokinase), secreted by the duodenal brush border, is the initial trigger that converts Trypsinogen to Trypsin. 2. **Pancreatitis Link:** Premature activation of trypsinogen within the pancreas (due to ductal obstruction or injury) leads to autodigestion of the gland, resulting in acute pancreatitis. 3. **Protective Mechanism:** The pancreas secretes **PSTI (Pancreatic Secretory Trypsin Inhibitor)** to prevent accidental internal activation of trypsin.

Q: Which one of the following is a lyase enzyme?

Aldolase B. **Explanation:** Enzymes are classified into six major classes based on the type of reaction they catalyze (IUBMB classification). **Lyases (Class 4)** are enzymes that catalyze the cleavage of C-C, C-O, C-N, or other bonds by means other than hydrolysis or oxidation, often resulting in the formation of a double bond or the addition of groups to double bonds. **Why Aldolase B is the correct answer:** Aldolase B (Fructose-1,6-bisphosphate aldolase) catalyzes the reversible cleavage of Fructose-1,6-bisphosphate into Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde-3-phosphate. In the liver, it also cleaves Fructose-1-phosphate. Since it breaks a C-C bond without the use of water or redox cofactor, it is a classic example of a **Lyase**. **Analysis of Incorrect Options:** * **Hexokinase (Option A):** This is a **Transferase (Class 2)**. It transfers a phosphate group from ATP to a hexose sugar (glucose). * **Pyruvate Kinase (Option B):** Despite the name "kinase," it is also a **Transferase (Class 2)**, transferring a phosphate group from phosphoenolpyruvate (PEP) to ADP. * **Propionyl CoA Carboxylase (Option C):** This is a **Ligase (Class 6)**. Carboxylases use ATP energy to join a CO₂ molecule to a substrate. **High-Yield Clinical Pearls for NEET-PG:** * **Hereditary Fructose Intolerance (HFI):** Caused by a deficiency of **Aldolase B**. It leads to the accumulation of Fructose-1-Phosphate, causing hypoglycemia and liver damage. * **Mnemonic for Enzyme Classes:** **"O T H L I L"** (Oxidoreductases, Transferases, Hydrolases, Lyases, Isomerases, Ligases). * **Synthases vs. Synthetases:** *Synthases* are Lyases (do not require ATP), whereas *Synthetases* are Ligases (require ATP).

Q: Enolase is inhibited by which of the following?

Fluoride. **Explanation:** **Enolase** is a key glycolytic enzyme that catalyzes the dehydration of 2-phosphoglycerate to phosphoenolpyruvate (PEP). It is a metalloenzyme that requires **Magnesium (Mg²⁺)** ions for its catalytic activity. 1. **Why Fluoride is Correct:** Fluoride acts as a potent competitive inhibitor of Enolase. It reacts with inorganic phosphate and magnesium to form a complex called **Magnesium-Fluorophosphate**. This complex binds to the active site of the enzyme, displacing the essential Mg²⁺ ions and effectively halting glycolysis. 2. **Why Other Options are Incorrect:** * **Fumarate:** This is an intermediate in the TCA cycle and not a classic enzyme inhibitor in this context. * **Iodoacetate:** This inhibits **Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)** by binding to the essential –SH (sulfhydryl) groups at its active site. * **Arsenite:** Trivalent arsenic (Arsenite) inhibits enzymes requiring **Lipoic acid** as a cofactor, such as the Pyruvate Dehydrogenase (PDH) complex and α-ketoglutarate dehydrogenase. **Clinical Pearls & High-Yield Facts for NEET-PG:** * **Blood Glucose Estimation:** In clinical practice, blood samples for glucose estimation are collected in **Fluoride bulbs (Grey top)** containing Sodium Fluoride (NaF) and Potassium Oxalate. NaF inhibits Enolase to prevent "in vitro" glycolysis by RBCs, ensuring the glucose level remains stable until analysis. * **Oxalate's Role:** While Fluoride inhibits glycolysis, Potassium Oxalate acts as an anticoagulant by chelating calcium. * **Arsenate vs. Arsenite:** Note that *Arsenate* (pentavalent) competes with inorganic phosphate in the GAPDH reaction, leading to the bypass of ATP synthesis (substrate-level phosphorylation), whereas *Arsenite* (trivalent) inhibits PDH.

Q: Odd chain fatty acids can form glucose by which pathway?

Formation of Propionyl CoA. **Explanation:** The correct answer is **A. Formation of Propionyl CoA.** **1. Why Option A is correct:** Most fatty acids are even-chained and break down into Acetyl CoA, which cannot be used for gluconeogenesis because the PDH reaction is irreversible. However, **odd-chain fatty acids** undergo beta-oxidation to yield Acetyl CoA units plus a final 3-carbon fragment called **Propionyl CoA**. Propionyl CoA is converted into **Succinyl CoA** (via Propionyl CoA carboxylase and Methylmalonyl CoA mutase), which enters the TCA cycle. Since Succinyl CoA can be converted to Oxaloacetate (OAA) and subsequently enter the gluconeogenic pathway, odd-chain fatty acids are considered **glucogenic**. **2. Why other options are incorrect:** * **Option B (Glycerol):** While glycerol (from triglyceride breakdown) can form glucose, it is a separate component from the fatty acid chains themselves. The question specifically asks about the fatty acid pathway. * **Option C (Acetyl CoA):** Acetyl CoA enters the TCA cycle but is completely oxidized to $CO_2$. There is no net synthesis of glucose from Acetyl CoA in humans. * **Option D (Lactic acid):** Lactic acid is a product of anaerobic glycolysis (Cori cycle) and is not a product of fatty acid oxidation. **High-Yield Clinical Pearls for NEET-PG:** * **Vitamin B12 Connection:** The conversion of Methylmalonyl CoA to Succinyl CoA requires **Vitamin B12**. Deficiency leads to Methylmalonic aciduria. * **Biotin Connection:** Propionyl CoA carboxylase requires **Biotin (B7)**. * **Key Concept:** Odd-chain fatty acids are the *only* lipids that can contribute to a net gain of glucose (excluding the glycerol backbone).

Q: Arrange the following enzyme categories as per increasing order of their enzyme commission (EC) numbers?

Transferases - Hydrolases - Hydratase - Isomerases. To master enzyme classification for NEET-PG, remember the standard mnemonic **"O T H L I L"**, which represents the six major classes of enzymes in their specific numerical order (EC 1 to EC 6). ### 1. Understanding the Correct Sequence (Option B) The Enzyme Commission (EC) numbers are assigned based on the type of reaction catalyzed: * **EC 1: Oxidoreductases** (Redox reactions) * **EC 2: Transferases** (Transfer of functional groups) * **EC 3: Hydrolases** (Cleavage of bonds using water) * **EC 4: Lyases** (Addition/removal of groups to form double bonds; includes **Hydratases**) * **EC 5: Isomerases** (Intramolecular rearrangements) * **EC 6: Ligases** (Joining two molecules using ATP) In the correct option (B), the sequence follows: **Transferases (2) < Hydrolases (3) < Hydratase (a subclass of Lyases, 4) < Isomerases (5)**. This perfectly matches the increasing numerical order. ### 2. Analysis of Incorrect Options * **Option A:** Starts with Isomerases (5), which is higher than Transferases (2). * **Option C:** Starts with Hydratase (4), placing it before Transferases (2) and Hydrolases (3). * **Option D:** Places Hydrolases (3) before Transferases (2). ### 3. High-Yield Clinical Pearls for NEET-PG * **Hydratase vs. Hydrolase:** Do not confuse them. **Hydrolases (EC 3)** use water to break a bond (e.g., Pepsin), while **Hydratases (EC 4)** add or remove water without breaking the primary skeleton (e.g., Enolase, Fumarase). * **Kinases:** These belong to **Transferases (EC 2)** because they transfer a phosphate group from ATP. * **Dehydrogenases:** These are the most common **Oxidoreductases (EC 1)**. * **Transaminases (ALT/AST):** These are **Transferases (EC 2)** and are vital markers for liver injury.

Q: All of the following statements about functional enzymes are TRUE, EXCEPT:

Substrates for functional enzymes are absent in blood.. ### Explanation The question asks for the **incorrect** statement regarding functional enzymes. **1. Why Option C is the Correct Answer (The False Statement):** Functional plasma enzymes are those that have a specific physiological role in the blood. For these enzymes to perform their functions, their **substrates must be present in the blood**. For example, the substrate for thrombin (fibrinogen) is always present in the plasma to facilitate coagulation. Therefore, stating that substrates are absent is factually incorrect. **2. Analysis of Other Options:** * **Option A (True):** Prothrombin (Factor II) is a classic example of a functional enzyme. Other examples include Lipoprotein Lipase (LPL) and enzymes of the complement system. * **Option B (True):** Functional enzymes are actively secreted into the blood by organs (like the liver) and maintain a **higher concentration in the plasma** than in the tissues. This is the opposite of *non-functional* enzymes (like ALT or CK), which are intracellular and only appear in high plasma levels during tissue damage. * **Option D (True):** Since most functional enzymes (like clotting factors) are synthesized in the **liver**, their activity and concentration significantly **decrease in liver disease**, leading to clinical issues like coagulopathy. **3. High-Yield Clinical Pearls for NEET-PG:** * **Functional Enzymes:** Synthesized in the liver, active in plasma, substrates present in blood. Examples: Clotting factors, Pseudocholinesterase, Lipoprotein lipase. * **Non-Functional Enzymes:** No physiological role in plasma, high concentration in tissues, low in plasma. Their rise indicates **cell death or membrane damage**. Examples: LDH, AST, ALT, Amylase. * **Diagnostic Tip:** A decrease in functional enzymes (e.g., low Prothrombin Time/INR) is a sensitive indicator of liver biosynthetic failure.

Question 1

Which enzyme is primarily stored in muscle?

Accepted Answer

SGOT

Answer

Alkaline phosphatase

Answer

SGPT

Answer

CPK

Question 2

Trypsin is an activator of all of the following enzymes EXCEPT?

Accepted Answer

Pepsinogen

Answer

Chymotrypsinogen

Answer

Proelastase

Answer

Procolipase

Question 3

Which of the following is true about ribozymes?

Accepted Answer

Deamination

Answer

Catalytic activity

Answer

Involved in transesterification

Answer

Hammerhead metalloenzyme

Question 4

A transporter has a Km for glucose of 45 mM and a Vmax of 36 micromoles glucose/sec/mg of transporter. If the glucose concentration in peripheral blood is 15 mM, what will the rate of glucose transport (in micromoles glucose/sec/mg transporter) be?

Accepted Answer

12

Answer

6

Answer

3

Answer

9

Question 5

Which of the following statements about enzyme kinetics is true?

Accepted Answer

In competitive inhibition, Vmax remains unchanged, but Km increases.

Answer

In uncompetitive inhibition, Km remains unchanged, but Vmax increases.

Answer

In uncompetitive inhibition, Km remains unchanged, but Vmax increases.

Answer

In uncompetitive inhibition, there is a mixed increase of both Vmax and Km values.

Question 6

Which one of the following is a lyase enzyme?

Accepted Answer

Aldolase B

Answer

Hexokinase

Answer

Pyruvate kinase

Answer

Proponyl CoA carboxylase

Question 7

Enolase is inhibited by which of the following?

Accepted Answer

Fluoride

Answer

Fumarate

Answer

Iodoacetate

Answer

Arsenite

Question 8

Odd chain fatty acids can form glucose by which pathway?

Accepted Answer

Formation of Propionyl CoA

Answer

Formation of Glycerol

Answer

Entry of Acetyl CoA into the TCA cycle

Answer

Lactic acid formation

Question 9

Arrange the following enzyme categories as per increasing order of their enzyme commission (EC) numbers?

Accepted Answer

Transferases - Hydrolases - Hydratase - Isomerases

Answer

Isomerases - Hydratase - Transferases - Hydrolases

Answer

Hydratase - Hydrolases - Transferases - Isomerases

Answer

Hydrolases - Transferases - Isomerases - Hydratase

Question 10

All of the following statements about functional enzymes are TRUE, EXCEPT:

Accepted Answer

Substrates for functional enzymes are absent in blood.

Answer

Prothrombin is a functional enzyme.

Answer

Functional enzymes are present in plasma in higher concentration than in tissue.

Answer

Functional enzyme activity is decreased in liver disease.

Enzymes — MCQs

Enzymes — MCQs

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