Molecular Diagnostic Methods Practice Questions

Question 1

What is Northern blot used to detect?

Accepted Answer

RNA

Answer

Protein

Answer

Immunoglobulin

Answer

DNA

Question 2

What is the best investigation for identifying malaria species?

Accepted Answer

Thin smear with Giemsa

Answer

Thick smear

Answer

QBC

Answer

Thin smear with acridine orange

Question 3

True about polymerase chain reaction is:

Accepted Answer

Enzymatic DNA amplification

Answer

Non-enzymatic DNA amplification

Answer

Recombinant DNA amplification

Answer

Separation of protein fragments in serum

Question 4

DNA sequence is determined by?

Accepted Answer

Sanger sequencing

Answer

PCR

Answer

FISH

Answer

Gel electrophoresis

Question 5

Which one of the following enzymes is obtained from Thermus aquaticus bacterium that is heat stable and used in PCR at high temperature?

Accepted Answer

Taq polymerase

Answer

DNA gyrase

Answer

DNA polymerase III

Answer

Endonuclease

Question 6

Which of the following statements about nucleic acid amplification tests (NAATs) for STIs is FALSE?

Accepted Answer

They are less sensitive than culture for rectal chlamydia

Answer

They can be used for test of cure after 3 weeks

Answer

They can detect dead organisms after treatment

Answer

They can be used for pharyngeal gonorrhea screening

Question 7

Which of the following is a xenodiagnostic method?

Accepted Answer

Injecting Aedes thorax with blood of a suspected dengue patient

Answer

Intradermal test on guinea pigs for toxigenicity of Corynebacterium diphtheriae

Answer

Injecting a hamster with splenic biopsy for diagnosis of leishmaniasis

Answer

Rabbit ileal loop for enterotoxigenic Escherichia coli

Question 8

A frequent traveler presented with 4 days of continuous fever, abdominal pain, and bradycardia. What is the best diagnostic test to confirm the pathogen?

Accepted Answer

Blood culture

Answer

Widal test

Answer

Urine culture

Answer

Stool culture

Question 9

Which of the following statements is MOST accurate regarding herpes encephalitis?

Accepted Answer

The temporal lobe is commonly involved.

Answer

Focal neurological symptoms are common.

Answer

EEG findings are nonspecific and not diagnostic.

Answer

MRI is a key diagnostic tool.

Question 10

Which of the following is not a diagnostic criterion for SIRS?

Accepted Answer

Hypotension

Answer

Tachypnoea

Answer

Leucocytosis

Answer

Tachycardia

Molecular Diagnostic Methods — MCQs

Molecular Diagnostic Methods — MCQs

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