DNA Profiling Practice Questions

Question 1

Which of the following techniques can be used to detect single base pair substitutions?

Accepted Answer

PCR

Answer

FISH

Answer

Southern blot

Answer

Restriction Fragment Length Polymorphism (RFLP)

Question 2

PCR is primarily a

Accepted Answer

DNA amplification technique

Answer

DNA sequencing technique

Answer

All of these

Answer

DNA degradation technique

Question 3

Mutations are due to changes in:

Accepted Answer

DNA nucleotide sequence

Answer

RNA nucleotide sequence

Answer

Amino acid sequence of ribonuclease

Answer

Cell membrane

Question 4

DNA sequence is determined by?

Accepted Answer

Sanger sequencing

Answer

PCR

Answer

FISH

Answer

Gel electrophoresis

Question 5

For DNA extraction from blood samples, the preferred anticoagulant is:

Accepted Answer

EDTA

Answer

Plain bulb

Answer

Formalin

Answer

None of the options

Question 6

Which of the following is used to detect abnormal gene sequences EXCEPT?

Accepted Answer

Flow cytometry

Answer

RFLP analysis

Answer

Pyrosequencing

Answer

FISH

Question 7

Gene amplification is achieved through

Accepted Answer

Polymerase Chain Reaction

Answer

DNA strand hybridization

Answer

In situ DNA hybridization

Answer

Ligase chain reaction (LCR)

Question 8

DNA fingerprinting is used for paternity testing and forensic identification of suspects. Which of the following is the most accurate description of DNA fingerprinting?

Accepted Answer

DNA can be isolated from blood, skin, or sperm and analyzed for variable patterns of restriction fragments arising from tandemly repeated sequences (microsatellites)

Answer

DNA is isolated from blood, skin, or sperm and its fragment size distribution is analyzed by gel electrophoresis

Answer

DNA is isolated from blood, skin, or sperm and hybridized with probes from the HLA locus to visualize HLA gene patterns

Answer

DNA is copied from blood, skin, or sperm RNA using reverse transcriptase and analyzed for the pattern of complementary DNAs

Question 9

Identical twins can be differentiated by their ________.

Accepted Answer

Fingerprint

Answer

Blood grouping

Answer

DNA fingerprinting

Answer

Age

Question 10

Which of the following statements best describes a key characteristic of fingerprint development?

Accepted Answer

Fingerprints are fully formed before birth.

Answer

DNA analysis is the most specific method of identification.

Answer

The most common type of fingerprint is loops.

Answer

None of the options are true.

DNA Profiling — MCQs

DNA Profiling — MCQs

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