Short Tandem Repeat Analysis Practice Questions

Question 1

Which of the following techniques can be used to detect single base pair substitutions?

Accepted Answer

PCR

Answer

FISH

Answer

Southern blot

Answer

Restriction Fragment Length Polymorphism (RFLP)

Question 2

Which of the following is the least suitable source for DNA extraction?

Accepted Answer

CSF

Answer

Hair roots

Answer

Semen

Answer

Buccal mucosa

Question 3

DNA fingerprinting was first used by Alec Jeffreys in a criminal case for detecting:

Accepted Answer

Rape

Answer

Immigration purpose

Answer

Disputed paternity

Answer

Murder

Question 4

DNA fingerprinting is used for paternity testing and forensic identification of suspects. Which of the following is the most accurate description of DNA fingerprinting?

Accepted Answer

DNA can be isolated from blood, skin, or sperm and analyzed for variable patterns of restriction fragments arising from tandemly repeated sequences (microsatellites)

Answer

DNA is isolated from blood, skin, or sperm and its fragment size distribution is analyzed by gel electrophoresis

Answer

DNA is isolated from blood, skin, or sperm and hybridized with probes from the HLA locus to visualize HLA gene patterns

Answer

DNA is copied from blood, skin, or sperm RNA using reverse transcriptase and analyzed for the pattern of complementary DNAs

Question 5

Which of the following are examples of trinucleotide repeat mutations?

Accepted Answer

All of the options

Answer

Friedreich ataxia

Answer

Fragile X syndrome

Answer

Huntington's chorea

Question 6

34 week primigravida punjabi khatri comes with history of consanguineous marriage, with history of repeated blood transfusion to her sibling since 8 months of age. The first diagnostic test is -

Accepted Answer

Hb electrophoresis

Answer

HPLC

Answer

Bone marrow

Answer

Blood smear

Question 7

The given karyotype is seen in which of the following syndromes?

Accepted Answer

Down syndrome

Answer

Angelman syndrome

Answer

Fragile x syndrome

Answer

Turner syndrome

Question 8

In a macerated baby, the ideal sample for genetic analysis is obtained from:

Accepted Answer

Placental Tissue

Answer

Clotted fetal blood

Answer

Fibroblast from skin

Answer

Fibroblast from Achilles tendon

Question 9

The Takayama test is primarily used for what purpose?

Accepted Answer

To determine the crystalline structure of a stain.

Answer

To identify the species of origin of a stain.

Answer

To perform blood grouping.

Answer

None of the above.

Question 10

What is a confirmatory test for species of origin?

Accepted Answer

Ouchterlony Test

Answer

Origin Test

Answer

Olympus Test

Answer

SOO Test

Short Tandem Repeat Analysis — MCQs

Short Tandem Repeat Analysis — MCQs

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