PCR Technology Practice Questions

Question 1

Steps of PCR in sequence are?

Accepted Answer

Denature DNA, Anneal Primers, Extend DNA

Answer

Denature DNA, Extend DNA, Anneal Primers

Answer

Anneal Primers, Extend DNA, Denature DNA

Answer

Extend DNA, Anneal Primers, Denature DNA

Question 2

Which of the following is a primarily RNA based technique?

Accepted Answer

Northern blotting

Answer

Western blotting

Answer

Southern blotting

Answer

Sanger's technique

Question 3

Which one of the following enzymes is obtained from Thermus aquaticus bacterium that is heat stable and used in PCR at high temperature?

Accepted Answer

Taq polymerase

Answer

DNA gyrase

Answer

DNA polymerase III

Answer

Endonuclease

Question 4

DNA amplification is done by all, except:

Accepted Answer

DNA sequencing

Answer

Loop-mediated isothermal amplification (LAMP)

Answer

Ligase chain reaction

Answer

Polymerase chain reaction

Question 5

What is the underlying principle of using mitochondrial DNA rather than nuclear DNA for identification in severely degraded disaster victim remains?

Accepted Answer

Mitochondrial DNA is present in thousands of copies per cell making it more likely to be recovered

Answer

Mitochondrial DNA is more specific for individual identification

Answer

Mitochondrial DNA is not affected by environmental factors

Answer

Mitochondrial DNA analysis is less expensive and faster

Question 6

Absolute proof of monozygosity is determined by?

Accepted Answer

DNA fingerprinting

Answer

Intervening membrane layers

Answer

Sex of the babies

Answer

Reciprocal skin grafting

Question 7

What is a confirmatory test for species of origin?

Accepted Answer

Ouchterlony Test

Answer

Origin Test

Answer

Olympus Test

Answer

SOO Test

Question 8

Which of the following is the best method for paternity testing?

Accepted Answer

Microsatellite analysis

Answer

Karyotyping

Answer

Northern blot analysis

Answer

All of the above

Question 9

What is the purpose of using a dried blood stain's crust in forensic analysis?

Accepted Answer

Determination of the blood group

Answer

Detection of the species of origin

Answer

Determination of secretor status

Answer

Characterization of the nature of the stain

Question 10

What is the most informative test for parental identification?

Accepted Answer

DNA fingerprinting

Answer

Human Leukocyte Antigen (HLA)

Answer

Parental likeness assessment

Answer

Assessment of developmental defects

PCR Technology — MCQs

PCR Technology — MCQs

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