DNA Structure and Function Practice Questions

Question 1

Which of the following techniques can be used to detect single base pair substitutions?

Accepted Answer

PCR

Answer

FISH

Answer

Southern blot

Answer

Restriction Fragment Length Polymorphism (RFLP)

Question 2

Mutations are due to changes in:

Accepted Answer

DNA nucleotide sequence

Answer

RNA nucleotide sequence

Answer

Amino acid sequence of ribonuclease

Answer

Cell membrane

Question 3

Which of the following is not a component of a nucleotide?

Accepted Answer

Fatty acid

Answer

Sugar

Answer

Base

Answer

Phosphate

Question 4

DNA sequence is determined by?

Accepted Answer

Sanger sequencing

Answer

PCR

Answer

FISH

Answer

Gel electrophoresis

Question 5

Mark the false statement regarding mitochondrial DNA:

Accepted Answer

Does not show heteroplasmy

Answer

AGA and AGG are stop codons in mitochondrial DNA

Answer

Kearns-Sayre Syndrome is a large deletion in mitochondrial DNA

Answer

1% of cellular DNA, 13 proteins of respiratory chain

Question 6

Which of the following is NOT a characteristic of the genetic code?

Accepted Answer

Overlapping

Answer

Universal

Answer

Degeneracy

Answer

Nonambiguous

Question 7

What sequence on the template strand of DNA corresponds to the first amino acid inserted into a protein?

Accepted Answer

3' TAC 5'

Answer

3' TAG 5'

Answer

3' TAA 5'

Answer

3' ATG 5'

Question 8

Which of the following statements about Taq DNA polymerase is correct?

Accepted Answer

Optimum temperature for chain elongation is 75°C

Answer

Denatures at high temperatures

Answer

Provides high fidelity during DNA synthesis

Answer

Exhibits 3' to 5' exonuclease activity

Question 9

Best sample for DNA profiling in sexual assault after 72 hours?

Accepted Answer

Victim's clothes

Answer

Cervical swab

Answer

Vaginal swab

Answer

Fingernail scrapings

Question 10

Best site for DNA extraction from a 2-month-old decomposed body?

Accepted Answer

Teeth

Answer

Muscle

Answer

Bone

Answer

Hair

DNA Structure and Function — MCQs

DNA Structure and Function — MCQs

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