Polymerase Chain Reaction (PCR) Practice Questions

Question 1

Which PCR technique is best suited for identifying a syndrome with multiple causative agents?

Accepted Answer

Multiplex PCR

Answer

RT-PCR

Answer

Nested PCR

Answer

Conventional PCR

Question 2

Steps of PCR in sequence are?

Accepted Answer

Denature DNA, Anneal Primers, Extend DNA

Answer

Denature DNA, Extend DNA, Anneal Primers

Answer

Anneal Primers, Extend DNA, Denature DNA

Answer

Extend DNA, Anneal Primers, Denature DNA

Question 3

Which of the following techniques is primarily used for RNA analysis?

Accepted Answer

Next generation sequencing

Answer

Sanger's technique

Answer

Western blot

Answer

PCR

Question 4

What is meant by the melting of double-stranded DNA?

Accepted Answer

Splitting of double strands into single strands

Answer

Splitting of DNA into fragments

Answer

Formation of triple helix

Answer

Separation of double-stranded bases

Question 5

Which of the following is a primarily RNA based technique?

Accepted Answer

Northern blotting

Answer

Western blotting

Answer

Southern blotting

Answer

Sanger's technique

Question 6

Which of the following statements about Taq DNA polymerase is correct?

Accepted Answer

Optimum temperature for chain elongation is 75°C

Answer

Denatures at high temperatures

Answer

Provides high fidelity during DNA synthesis

Answer

Exhibits 3' to 5' exonuclease activity

Question 7

DNA amplification is done by all, except:

Accepted Answer

DNA sequencing

Answer

Loop-mediated isothermal amplification (LAMP)

Answer

Ligase chain reaction

Answer

Polymerase chain reaction

Question 8

Which one of the following enzymes is obtained from Thermus aquaticus bacterium that is heat stable and used in PCR at high temperature?

Accepted Answer

Taq polymerase

Answer

DNA gyrase

Answer

DNA polymerase III

Answer

Endonuclease

Question 9

Gene amplification is achieved through

Accepted Answer

Polymerase Chain Reaction

Answer

DNA strand hybridization

Answer

In situ DNA hybridization

Answer

Ligase chain reaction (LCR)

Question 10

According to IUB system, hydrolases belong to which class?

Accepted Answer

EC-3

Answer

EC-1

Answer

EC-2

Answer

EC-4

Polymerase Chain Reaction (PCR) — MCQs

Polymerase Chain Reaction (PCR) — MCQs

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