DNA Sequencing Practice Questions

Question 1

Which of the following techniques is primarily used for RNA analysis?

Accepted Answer

Next generation sequencing

Answer

Sanger's technique

Answer

Western blot

Answer

PCR

Question 2

Mutations are due to changes in:

Accepted Answer

DNA nucleotide sequence

Answer

RNA nucleotide sequence

Answer

Amino acid sequence of ribonuclease

Answer

Cell membrane

Question 3

Restriction fragment length polymorphism is used for:

Accepted Answer

Genetic mapping and identification

Answer

Detection of gene mutations

Answer

Paternity testing

Answer

Forensic analysis

Question 4

Gene amplification is achieved through

Accepted Answer

Polymerase Chain Reaction

Answer

DNA strand hybridization

Answer

In situ DNA hybridization

Answer

Ligase chain reaction (LCR)

Question 5

Which of the following is the least suitable source for DNA extraction?

Accepted Answer

CSF

Answer

Hair roots

Answer

Semen

Answer

Buccal mucosa

Question 6

Which of the following doesn't occur in 5' to 3' direction?

Accepted Answer

RNA editing

Answer

DNA repair

Answer

Transcription

Answer

DNA replication

Question 7

What is the most important tool used in genetic engineering?

Accepted Answer

Restriction endonuclease

Answer

Topoisomerase

Answer

DNA Ligase

Answer

Helicase

Question 8

Which of the following techniques can be used to detect single base pair substitutions?

Accepted Answer

PCR

Answer

FISH

Answer

Southern blot

Answer

Restriction Fragment Length Polymorphism (RFLP)

Question 9

Which of the following is a primarily RNA based technique?

Accepted Answer

Next generation sequencing

Answer

Sanger's technique

Answer

Western blotting

Answer

PCR

Question 10

All are added to PCR, except:

Accepted Answer

Dideoxynucleotide

Answer

Thermostable DNA polymerase

Answer

Template DNA

Answer

Deoxynucleotide

DNA Sequencing — MCQs

DNA Sequencing — MCQs

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