Molecular Genetics — Flashcards

Lab animals deprived of folic acid experience a marked increase in marrow eythroid precursor cell production. -Many of these erythroid cells undergo apoptosis without further maturation. -Provision of what supplements would reduce erythroid precursor cell apoptosis in these animals?

What are the followins in relations to both eukaryotic and prokaryotic DNA? -Continuous synthesis of the lagging strand -Energy-independent DNA unwinding -Multiple origins of replication -No proofreading of daughter strands -No requirement for RNA primers

A researcher develops 2 functional mRNA sequences composed of CUC & CUU trinucleotide repeats. -He subsequntly incubates them. -After a few hours, it is found that both mRNA sequences produce polypeptide chains containing leucine repeats. -This observed finding is due to what genetic principles?

An infant born appears healthy at birth but develops transfusion-dependent hymolytic anemia by age of 6 months. -His erythrocytes contain insoluble aggregated of hemoglobin subunits. -The child developed normally in utero because at that time he produced high quantities of:

What are the followings are associated with? -Proteasome activity -Splicing

Match the following with their specific definition? -Unwinding of double helix -Removal of supercoils -Stabilization of unwound template strands -Synthesis of RNA primer -5' to 3 DNA synthesis & 3' to 5' exonuclease activity -Removes RNA primer -Has 5' to 3' exonuclease activity -Joining of Okazaki fragments -Similar work to DNA polymerase 3 -Connect the lagging strands together.

The following Hemoglobin are associted with: -Hemoglobin C -Hemoglobin F -Hemoglobin H -Hemoglobin S

34 y/o woman comes for breast examination. -Screening test was performed using Polymerase chain reaction (PCR) to amplify the target of exon. -Gel electrophoresis to assess the size of the exon compared to the wild type allele.What features must be known in order to perform the amplification part of this analysis?

HBC disease is caused by a single amino acid substitution (glutamic acid ---> lysine) at position 6 in B-globin chain of Hb molecule. -Patient homozygous for HBC have mild chronic hemolytic anemia. -Those with HbS generally have a more severe condition. -Which properties of HbS best explains why HbS disease is associated with more pronouced clinical manifestations than HbC disease?

A 2 y/o boy comes with easy bruising. -Parent stated he develops marked bruising & open wounds following minor trauma. -Skin is difficult to suture due to its extreme fragility. -Physical exam: Hyperextensible skin, multiple ecchymoses over the forearms. - Skin biopsy: defect in extracellular processing of collagen. -Which steps of collagen synthesis is most likely impaired in this patient?