Molecular Genetics — Flashcards

Flashcard 1: What triplet is associated with Huntington's disease?

Answer: CAG mnemonic: X-Girlfriend's First Aid Helped Ace My Test

Flashcard 2: What triplet is associated with Fragile X syndrome?

Answer: CGG mnemonic: X-Girlfriend's First Aid Helped Ace My Test

Flashcard 3: What triplet is associated with myotonic dystrophy?

Answer: CTG mnemonic: X-Girlfriend's First Aid Helped Ace My Test

Flashcard 4: Uniparental isodisomy indicates an error in what process?

Answer: meoisis II or postzygotic duplication

Flashcard 5: Southwestern

Answer: DNA-binding proteins

Extra: labeled oligonucleotide

Flashcard 6: Southern

Answer: DNA

Extra: complementary labeled DNA

Flashcard 7: Explain the process for a molecular blot. (any type)

Answer: 1. sample is electrophoresed on gel 2. sample transfered to filter paper 3. sample exposed to labeled probe; probe binds sample 4. banding pattern visualized with probe

Flashcard 8: How does an indirect enzyme-linked immunosorbent assay (ELISA) work?

Answer: 1. Patient's blood sample washed over a fixed antigen. 2. Antigen field is washed with anti-human antibody linked to a color-generating enzyme. 2. Any antigen-antibody-antibody-enzyme complex produces color change.

Flashcard 9: What occurs during the exocytosis step of collagen synthesis?

Answer: procollagen triple helix is extruded into extracellular space

Flashcard 10: List the steps of PCR.

Answer: 1. Denaturation 2. Annealing 3. Elongation