Diagnostic Microbiology — Flashcards

Flashcard 21: Diagnosis of rickettsial diseases is by _____

Answer: Weil-Felix reaction

Extra: The Weil-Felix reaction is a heterophile agglutination test that uses Proteus antigens (OX-19, OX-2, and OX-K) to detect antibodies against Rickettsia. It is negative in Q fever (Coxiella burnetii).

Flashcard 22: _____ test is done to rule out Klebsiella in coliform test for E. coli

Answer: Eijkman

Flashcard 23: A direct ELISA tests for an _____, whereas the indirect ELISA tests for an antibody against an antigen

Answer: antigen

Flashcard 24: Bronchoalveolar lavage of _____ infection will reveal 'disk-shaped yeast' or cysts containing dark oval bodies

Answer: Pneumocystis jiroveci

Flashcard 25: _____ test is used to detect the polysaccharide capsular antigen present in Cryptococci

Answer: Latex agglutination

Flashcard 26: _____ and Urine rapid antigen tests can also be used to detect Histoplasma

Answer: Serum

Flashcard 27: _____ reaction is used for detection of lecithinase toxin produced by Clostridium perfringens.

Answer: Nagler

Flashcard 28: _____ requires light microscope for interpretation whereas RPR can be read macroscopically (VDRL/RPR)

Answer: VDRL

Flashcard 29: _____ Media is a selective media that only grows Neisseria

Answer: Thayer-Martin

Flashcard 30: According to Ridley's logarithmic scale for bacterial index, _____ rating is 10-100 bacilli, on average, in each oil-immersion field

Answer: 4+