RNA Processing and Splicing — Flashcards

Flashcard 1: Acetylation and Deacetylation of _____ histones is associated with the activation or inactivation of gene transcription.

Answer: H3 and H4

Flashcard 2: _____ is the branch of molecular biology that deals with the study of heritable changes in gene function not involving changes in the DNA sequence.

Answer: Epigenetics

Flashcard 3: At its core, CRISPR/Cas9 utilizes an endonuclease (_____) and a guide RNA that binds to a complementary target DNA sequence

Answer: Cas9

Flashcard 4: A _____ blot is used to analyze RNA

Answer: Northern

Flashcard 5: _____ is a method that is used for analysis of mRNA expression and its amplification.

Answer: RT-PCR

Flashcard 6: In the final step of alternative splicing, the _____ is released to precisely remove the intron and join two exons

Answer: lariat

Flashcard 7: First step of initiation of protein synthesis (translation) involves _____ of the ribosome

Answer: dissociation into subunits

Flashcard 8: FISH may be carried out in Metaphase or _____phase of the cell cycle.

Answer: Inter

Flashcard 9: _____ test is a widely used biological assay to assess the mutagenic potential of chemical compounds.

Answer: Ames

Flashcard 10: There are approximately _____ genes present in the human genome.

Answer: 20,000-25,000

Extra: The number of protein-coding genes in the human genome was initially estimated by the Human Genome Project to be around 30,000-40,000, but has since been refined to approximately 20,000-25,000.