Gene Therapy Approaches — Flashcards

Flashcard 1: Blue-white assay used in the screening of recombinant vectors for inserted DNA fragments, is based on the principle of _____

Answer: complementation

Flashcard 2: _____ is the branch of molecular biology that deals with the study of heritable changes in gene function not involving changes in the DNA sequence.

Answer: Epigenetics

Flashcard 3: At its core, CRISPR/Cas9 utilizes an endonuclease (_____) and a guide RNA that binds to a complementary target DNA sequence

Answer: Cas9

Flashcard 4: A _____ is bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal.

Answer: genomic library

Flashcard 5: Chimeric DNA (also known as recombinant DNA) is used in _____

Answer: Genetic engineering

Flashcard 6: _____ assay is used in the screening of recombinant vectors for inserted DNA fragments

Answer: Blue-white

Flashcard 7: _____ mosaicism occurs when a mutation arises from mitotic errors after fertilization and propagates through multiple tissues or organs

Answer: Somatic

Flashcard 8: FISH may be carried out in Metaphase or _____phase of the cell cycle.

Answer: Inter

Flashcard 9: _____ test is a widely used biological assay to assess the mutagenic potential of chemical compounds.

Answer: Ames

Flashcard 10: There are approximately _____ genes present in the human genome.

Answer: 20,000-25,000

Extra: The number of protein-coding genes in the human genome was initially estimated by the Human Genome Project to be around 30,000-40,000, but has since been refined to approximately 20,000-25,000.