Epigenetics and DNA Methylation — Flashcards

Flashcard 1: Acetylation and Deacetylation of _____ histones is associated with the activation or inactivation of gene transcription.

Answer: H3 and H4

Flashcard 2: Histone _____ usually activates DNA transcription

Answer: acetylation

Flashcard 3: HDAC inhibitors prevent histones from being _____ resulting in genes such as tumor suppressors being transcribed

Answer: deacetylated

Flashcard 4: The promoter is an AT-rich upstream sequence with _____ and CAAT boxes

Answer: TATA

Flashcard 5: RET proto-oncogene is present on chromosome _____

Answer: 10

Flashcard 6: ADP-ribosylation of histones is associated with _____ and is carried out by vitamin B3

Answer: DNA repair

Flashcard 7: FISH may be carried out in Metaphase or _____phase of the cell cycle.

Answer: Inter

Flashcard 8: _____ test is a widely used biological assay to assess the mutagenic potential of chemical compounds.

Answer: Ames

Flashcard 9: There are approximately _____ genes present in the human genome.

Answer: 20,000-25,000

Extra: The number of protein-coding genes in the human genome was initially estimated by the Human Genome Project to be around 30,000-40,000, but has since been refined to approximately 20,000-25,000.

Flashcard 10: DNA _____ at CpG islands represses transcription

Answer: methylation