Chapter·MicrobiologyMicrobial genetics and drug resistance

MRSA resistance mechanismsDownloads

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1

A 37-year-old woman with a history of anorectal abscesses complains of pain in the perianal region. Physical examination reveals mild swelling, tenderness, and erythema of the perianal skin. She is prescribed oral ampicillin and asked to return for follow-up. Two days later, the patient presents with a high-grade fever, syncope, and increased swelling. Which of the following would be the most common mechanism of resistance leading to the failure of antibiotic therapy in this patient?

AIntrinsic absence of a target site for the drug

BUse of an altered metabolic pathway

CProduction of beta-lactamase enzyme

DAltered structural target for the drug

EDrug efflux pump

2

A 31-year-old female with a bacterial infection is prescribed a drug that binds the dipeptide D-Ala-D-Ala. Which of the following drugs was this patient prescribed?

APolymyxin B

BNalidixic acid

CChloramphenicol

DVancomycin

EPenicillin

3

A scientist is studying the mechanisms by which bacteria become resistant to antibiotics. She begins by obtaining a culture of vancomycin-resistant Enterococcus faecalis and conducts replicate plating experiments. In these experiments, colonies are inoculated onto a membrane and smeared on 2 separate plates, 1 containing vancomycin and the other with no antibiotics. She finds that all of the bacterial colonies are vancomycin resistant because they grow on both plates. She then maintains the bacteria in liquid culture without vancomycin while she performs her other studies. Fifteen generations of bacteria later, she conducts replicate plating experiments again and finds that 20% of the colonies are now sensitive to vancomycin. Which of the following mechanisms is the most likely explanation for why these colonies have become vancomycin sensitive?

APoint mutation

BGain of function mutation

CViral infection

DPlasmid loss

ELoss of function mutation

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