Chapter·MicrobiologyBiofilms

Detection methods for biofilmsDownloads

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Sample Questions

1

The surgical equipment used during a craniectomy is sterilized using pressurized steam at 121°C for 15 minutes. Reuse of these instruments can cause transmission of which of the following pathogens?

ANon-enveloped viruses

BSporulating bacteria

CPrions

DEnveloped viruses

EYeasts

2

A 24-year-old woman presents to the ED with symptoms of pelvic inflammatory disease despite being previously treated with azithromycin for chlamydial infection. Based on your clinical understanding about the epidemiology of PID, you decide to obtain a gram stain which shows a gram-negative diplococci. What is the next step in order to confirm the identity of the organism described?

APerform an RT-PCR

BCulture in TCBS agar

CCulture in Thayer-Martin media

DObtain an acid fast stain

ECulture in Bordet-Gengou agar

3

An investigator is studying bacterial toxins in a nonpathogenic bacterial monoculture that has been inoculated with specific bacteriophages. These phages were previously cultured in a toxin-producing bacterial culture. After inoculation, a new toxin is isolated from the culture. Genetic sequencing shows that the bacteria have incorporated viral genetic information, including the gene for this toxin, into their genome. The described process is most likely responsible for acquired pathogenicity in which of the following bacteria?

AStaphylococcus aureus

BHaemophilus influenzae

CNeisseria meningitidis

DStreptococcus pneumoniae

ECorynebacterium diphtheriae

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