Chapter·BiochemistryTranscription/translation

RNA interference and microRNAsDownloads

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Sample Questions

1

A 25-year-old female comes to the clinic complaining of fatigue and palpitations. She has been undergoing immense stress from her thesis defense and has been extremely tired. The patient denies any weight loss, diarrhea, cold/heat intolerance. TSH was within normal limits. She reports a family history of "blood disease" and was later confirmed positive for β-thalassemia minor. It is believed that abnormal splicing of the beta globin gene results in β-thalassemia. What is removed during this process that allows RNA to be significantly shorter than DNA?

A3'-poly(A) tail

BExons

CIntrons

DmicroRNAs

EsnRNPs

2

In translation, the wobble phenomenon is best illustrated by the fact that:

ACharged tRNA contains energy needed for peptide bonds to form

BThe last nucleotide provides specificity for the given amino acid

CA tRNA with the UUU anticodon can bind to either AAA or AAG codons

DThere are more amino acids than possible codons

EThe genetic code is preserved without mutations

3

A pharmaceutical company has modified one of its existing antibiotics to have an improved toxicity profile. The new antibiotic blocks protein synthesis by first entering the cell and then binding to active ribosomes. The antibiotic mimics the structure of aminoacyl-tRNA. The drug is covalently bonded to the existing growing peptide chain via peptidyl transferase, thereby impairing the rest of protein synthesis and leading to early polypeptide truncation. Where is the most likely site that this process occurs?

AE site

B30S small subunit

CA site

D40S small subunit

EP site

+ 7 more in the PDF

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