Chapter·MicrobiologyMicrobial genetics and drug resistance

Beta-lactamase types and mechanismsDownloads

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Sample Questions

1

A 37-year-old woman with a history of anorectal abscesses complains of pain in the perianal region. Physical examination reveals mild swelling, tenderness, and erythema of the perianal skin. She is prescribed oral ampicillin and asked to return for follow-up. Two days later, the patient presents with a high-grade fever, syncope, and increased swelling. Which of the following would be the most common mechanism of resistance leading to the failure of antibiotic therapy in this patient?

AIntrinsic absence of a target site for the drug

BUse of an altered metabolic pathway

CProduction of beta-lactamase enzyme

DAltered structural target for the drug

EDrug efflux pump

2

A 42-year-old woman with a history of multiple sclerosis and recurrent urinary tract infections comes to the emergency department because of flank pain and fever. Her temperature is 38.8°C (101.8°F). Examination shows left-sided costovertebral angle tenderness. She is admitted to the hospital and started on intravenous vancomycin. Three days later, her symptoms have not improved. Urine culture shows growth of Enterococcus faecalis. Which of the following best describes the most likely mechanism of antibiotic resistance in this patient?

AIncreased efflux across bacterial cell membranes

BProduction of beta-lactamase

CAlteration of penicillin-binding proteins

DAlteration of peptidoglycan synthesis

EAlteration of ribosomal targets

3

An 18-year old college freshman presents to his university clinic because he has not been feeling well for the past two weeks. He has had a persistent headache, occasional cough, and chills without rigors. The patient’s vital signs are normal and physical exam is unremarkable. His radiograph shows patchy interstitial lung infiltrates and he is diagnosed with atypical pneumonia. The patient is prescribed azithromycin and takes his medication as instructed. Despite adherence to his drug regimen, he returns to the clinic one week later because his symptoms have not improved. The organism responsible for this infection is likely resistant to azithromycin through which mechanism?

AMutation in topoisomerase II

BMethylation of ribosomal binding site

CPresence of a beta-lactamase

DDecreased binding to RNA polymerase

EInsertion of drug efflux pumps

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