Which is the correct sequence of steps in isolating desirable protein using recombinant DNA technology? 1. Expression of protein and lysis of the bacterial cell 2. Incorporation of genes into bacteria 3. SDS PAGE 4. Protein elution 5. Column chromatography
A2,1,3,5,4
B2,4,5,3,1
C1,2,4,3,5
D1,5,2,4,3
Which of the following statements best describes the mechanism of action of insulin on target cells?
AInsulin binds to a receptor on the outer surface of the plasma membrane, activating adenylate cyclase through the Gs protein.
BInsulin binds to a cytoplasmic receptor and is transferred as a hormone receptor complex to the nucleus to modulate gene expression.
CInsulin enters the cell and causes the release of calcium ions from intracellular stores.
DInsulin binds to a transmembrane receptor on the outer surface of the plasma membrane, activating the tyrosine kinase in the cytosolic domain of the receptor.
Which of the following is the platinum-based chemotherapeutic agent used as first-line treatment for ovarian carcinoma?
ACyclophosphamide
BMethotrexate
CCisplatin
DDacarbazine
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