Chapter·PathologyMolecular Pathology

Next-Generation SequencingDownloads

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Sample Questions

1

Which is the correct sequence of steps in isolating desirable protein using recombinant DNA technology? 1. Expression of protein and lysis of the bacterial cell 2. Incorporation of genes into bacteria 3. SDS PAGE 4. Protein elution 5. Column chromatography

A2,1,3,5,4

B2,4,5,3,1

C1,2,4,3,5

D1,5,2,4,3

2

Steps of PCR in sequence are?

ADenature DNA, Extend DNA, Anneal Primers

BAnneal Primers, Extend DNA, Denature DNA

CExtend DNA, Anneal Primers, Denature DNA

DDenature DNA, Anneal Primers, Extend DNA

3

Mutations are due to changes in:

ADNA nucleotide sequence

BRNA nucleotide sequence

CAmino acid sequence of ribonuclease

DCell membrane

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