General Pathology Practice Questions

Q: Which of the following types of cell death involves activation of caspase enzymes?

Apoptosis and Pyroptosis. ***Apoptosis and Pyroptosis*** - **Apoptosis** is the classic caspase-dependent programmed cell death pathway [1] - Involves **initiator caspases** (Caspase-8, Caspase-9) and **executioner caspases** (Caspase-3, -6, -7) - Characterized by cell shrinkage, chromatin condensation, and apoptotic body formation - **Pyroptosis** is an inflammatory form of programmed cell death that is strictly caspase-dependent [2] - Mediated by **inflammatory caspases** (Caspase-1, -4, -5, -11) [2] - Involves cleavage of Gasdermin-D leading to membrane pore formation - Triggered by inflammasome activation (particularly NLRP3) *Necrosis and Apoptosis* - **Necrosis** is caspase-independent and represents unregulated cell death from acute injury - Characterized by cell swelling, membrane rupture, and inflammatory response - Does not involve programmed activation of caspase enzymes *Apoptosis and necroptosis* - **Necroptosis** is a regulated form of necrotic cell death that occurs in a **caspase-independent** manner [2] - Executed through RIPK1-RIPK3-MLKL kinase pathway [2] - Typically occurs when caspase activity is inhibited or blocked *Apoptosis only* - Incomplete answer as **pyroptosis** is also inherently caspase-dependent - Pyroptosis uses inflammatory caspases (Caspase-1/4/5) distinct from apoptotic caspases - Both pathways represent distinct forms of caspase-mediated programmed cell death **References:** [1] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Cellular Responses to Stress and Toxic Insults: Adaptation, Injury, and Death, pp. 64-67. [2] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Cellular Responses to Stress and Toxic Insults: Adaptation, Injury, and Death, p. 71.

Q: Identify the tissue shown in the histopathological image:

Thymus. ***Thymus*** - The image shows a **thymic cortical area** characterized by a high density of lymphocytes (thymocytes) and Hassall's corpuscles [2]. - **Hassall's corpuscles**, visible as pink-staining, whorled epithelial structures are pathognomonic for the thymus [1]. *Lymph node* - Lymph nodes contain distinct cortical and medullary regions with **lymphoid follicles**, germinal centers, and sinuses filled with lymphocytes and macrophages. - They lack the characteristic **Hassall's corpuscles** seen in the image. *Tonsil* - Tonsils are characterized by crypts and germinal centers within lymphoid follicles, often covered by **stratified squamous epithelium**. - While they are lymphoid organs, they do not contain **Hassall's corpuscles**. *Spleen* - The spleen has a distinct **red pulp** (involved in blood filtration) and **white pulp** (lymphoid tissue around arterioles). - It does not contain Hassall's corpuscles and its architecture is markedly different from the image provided. **References:** [1] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Diseases of White Blood Cells, Lymph Nodes, Spleen, and Thymus, p. 634. [2] Cross SS. Underwood's Pathology: A Clinical Approach. 6th ed. Common Clinical Problems From Diseases Of The Urinary And Male Genital Tracts, pp. 572-574.

Q: All are true about the cartilage shown in the figure except: (Recent NEET Pattern 2016-17)

Present in intervertebral disks. ***Present in intervertebral disks*** ✓ Correct Answer (FALSE Statement) - The image shows **hyaline cartilage**, characterized by isolated chondrocytes (or in isogenous groups) within lacunae and a homogenous, glassy matrix. - **Intervertebral discs** are primarily composed of **fibrocartilage** (annulus fibrosus) with a gelatinous nucleus pulposus, NOT hyaline cartilage. - This is the **FALSE statement**, making it the correct answer to this "EXCEPT" question. *Hyaline cartilage with chondrocytes in lacunae* (TRUE Statement) - This statement is **true** about the cartilage shown. - The image clearly depicts **chondrocytes** residing within small spaces called **lacunae** embedded in an extracellular matrix [1]. - The matrix around the chondrocytes appears relatively uniform, which is characteristic of **hyaline cartilage**. *Ossifies with ageing* (TRUE Statement) - This is **true** for hyaline cartilage, especially articular cartilage and the cartilage of the nasal septum, larynx, and trachea. - Over time, in a process known as **endochondral ossification**, hyaline cartilage can be replaced by bone, leading to a decrease in flexibility [1]. *Most abundant cartilage in body* (TRUE Statement) - This statement is **true**. **Hyaline cartilage** is the most prevalent type of cartilage in the human body. - It is found in various locations such as the **articular surfaces of joints**, the **trachea**, bronchi, larynx, costal cartilages, and the embryonic skeleton. **References:** [1] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Bones, Joints, and Soft Tissue Tumors, pp. 1195-1204.

Q: A 6-month-old child presents with loss of vision and regression of milestones. On examination hepatosplenomegaly and lymphadenopathy is seen. The bone marrow aspiration shows abnormal macrophage shown below. It stains positive for fat and negative for iron. Identify the cell.

Niemann-Pick cell. ***Niemann-Pick cell*** - The image shows a macrophage with a **foamy, vacuolated cytoplasm**, which is characteristic of a Niemann-Pick cell, resulting from the accumulation of **sphingomyelin**. [1] - The clinical presentation of a 6-month-old with **loss of vision, regression of milestones, hepatosplenomegaly, and lymphadenopathy** is highly consistent with Niemann-Pick disease. [1] *Gaucher cell* - A Gaucher cell typically has a **fibrillar or "crinkled paper" cytoplasm** appearance due to the accumulation of glucocerebroside, which is different from the foamy appearance seen here. - While Gaucher disease presents with hepatosplenomegaly, **neurological regression and vision loss in infancy** are more characteristic of Niemann-Pick disease type A in this age group. *Hemosiderosis* - Hemosiderosis involves the accumulation of **iron (hemosiderin)** within macrophages, which would stain positive for iron. The question states the cells stained **negative for iron**. - Clinically, hemosiderosis is primarily characterized by organ damage due to iron overload, not neurological regression or vision loss in infancy as described. *Hallervorden Spatz disease* - Hallervorden Spatz disease (now known as pantothenate kinase-associated neurodegeneration) is a neurological disorder characterized by **iron accumulation in the basal ganglia**. - It does not primarily present with generalized macrophage abnormalities like those seen here, nor with prominent hepatosplenomegaly or lymphadenopathy and the affected cells would contain iron, not fat. **References:** [1] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Genetic Disorders, pp. 161-162.

Q: Agarose gel electrophoresis from DNA of a population of cells as seen under ultraviolet light is shown below. What is the correct explanation for the finding seen in the band labeled as "C"?

Apoptotic cells. ***Apoptotic cells*** - Band C shows a characteristic **DNA ladder pattern** with discrete bands representing fragmentation into nucleosome-sized units (multiples of ~180-200 base pairs), which is pathognomonic of **apoptosis**. [1] - During apoptosis, **endonucleases** cleave DNA at internucleosomal linker regions, creating uniform fragments that migrate as distinct bands on agarose gel electrophoresis. *Predominantly necrotic cells* - **Necrotic cell DNA** undergoes random, non-specific degradation by cellular enzymes, resulting in a continuous **smear pattern** rather than discrete bands. - The **smear appearance** reflects DNA fragments of varying sizes distributed throughout the gel, unlike the organized pattern seen in band C. *Mixed population of normal and apoptotic cells* - A mixed population would show both **intact high molecular weight DNA** (remaining near the gel origin) and the **apoptotic ladder pattern** superimposed. - Band C displays a pure ladder pattern without evidence of intact DNA, indicating a homogeneous apoptotic population. *A population of viable cells* - **Viable cells** maintain intact genomic DNA that remains as a single **high molecular weight band** near the top of the gel. - The **fragmented ladder pattern** in band C is incompatible with viable cell DNA, which should show minimal degradation. **References:** [1] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Cellular Responses to Stress and Toxic Insults: Adaptation, Injury, and Death, pp. 63-64.

Q: Identify the intracellular hyaline body.

Russell bodies. ***Russell bodies*** - The image depicts **plasma cells** containing large, eosinophilic, and homogenous cytoplasmic inclusions, which are characteristic features of **Russell bodies**. - These bodies represent an accumulation of **excess immunoglobulins** within the dilated cisternae of the endoplasmic reticulum in plasma cells [1]. *Corpora amylacea* - These are small, concentrically layered, usually **basophilic bodies** found extracellularly in the prostate, brain, and lung. - They are composed of glycoproteins and often increase with age, not directly seen here as intracellular hyaline bodies in plasma cells. *Hyaline droplets* - These are typically **intracellular protein reabsorption droplets** found in epithelial cells, particularly renal tubular cells, when there is excessive proteinuria [1]. - They are generally smaller and more uniformly distributed compared to Russell bodies and are not pathognomonic for plasma cells. *Mallory Hyaline bodies* - Also known as Mallory bodies, these are **eosinophilic, rope-like cytoplasmic inclusions** found in the hepatocytes of patients with alcoholic liver disease or certain other liver conditions [1]. - They are composed of intermediate filaments and are distinct from the immunoglobulin inclusions seen in plasma cells. **References:** [1] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. Cellular Responses to Stress and Toxic Insults: Adaptation, Injury, and Death, pp. 73-74.

Q: Ischemia-Reperfusion syndrome is characterized by:

Hypoxia and activation of inflammation. **Hypoxia and activation of inflammation** - **Ischemia-reperfusion injury** occurs when blood flow is restored to tissues previously deprived of oxygen, leading to further damage due to the rapid influx of oxygen and inflammatory mediators. - The initial **hypoxia** during ischemia triggers cellular changes, and subsequent reperfusion activates a robust **inflammatory response**, including the recruitment of neutrophils and the release of reactive oxygen species [1]. *Thrombo embolic angiopathy* - While thrombus formation can be a cause of ischemia, **thromboembolic angiopathy** itself is not the primary characteristic of the ischemia-reperfusion syndrome. - The syndrome's defining feature is the injury that occurs *after* the initial ischemic event, upon restoration of blood flow. *Build up of bicarbonate and Na+ ions* - Ischemia typically leads to a buildup of **lactic acid** and a decrease in pH (acidosis), not bicarbonate. - While electrolyte imbalances can occur, a specific buildup of bicarbonate and Na+ ions is not a hallmark characteristic of ischemia-reperfusion injury. *Acute mesenteric thrombosis* - **Acute mesenteric thrombosis** is a *cause* of mesenteric ischemia, not a general characteristic of the ischemia-reperfusion syndrome itself. - The syndrome describes the cellular and tissue injury that results from the initial ischemia and subsequent reperfusion, regardless of the underlying cause of ischemia. **References:** [1] Kumar V, Abbas AK, et al.. Robbins and Cotran Pathologic Basis of Disease. 9th ed. The Gastrointestinal Tract, pp. 786-787.

Question 1

Which of the following is a change seen in irreversible cell injury?

Accepted Answer

Amorphous density of mitochondria

Answer

Cellular Swelling

Answer

Bleb formation

Answer

Clumping of chromatin

Question 2

Which of the following types of cell death involves activation of caspase enzymes?

Accepted Answer

Apoptosis and Pyroptosis

Answer

Necrosis and Apoptosis

Answer

Apoptosis only

Answer

Apoptosis and necroptosis

Question 3

Identify the tissue shown in the histopathological image:

Accepted Answer

Thymus

Answer

Lymph node

Answer

Tonsil

Answer

Spleen

Question 4

All are true about the cartilage shown in the figure except: (Recent NEET Pattern 2016-17)

Accepted Answer

Present in intervertebral disks

Answer

Hyaline cartilage with chondrocytes in lacunae

Answer

Ossifies with ageing

Answer

Most abundant cartilage in body

Question 5

A 6-month-old child presents with loss of vision and regression of milestones. On examination hepatosplenomegaly and lymphadenopathy is seen. The bone marrow aspiration shows abnormal macrophage shown below. It stains positive for fat and negative for iron. Identify the cell.

Accepted Answer

Niemann-Pick cell

Answer

Gaucher cell

Answer

Hemosiderosis

Answer

Hallervorden-Spatz disease

Question 6

A karyotype is shown in the image. What is the most likely diagnosis?

Accepted Answer

Klinefelter syndrome

Answer

Turner syndrome

Answer

Down syndrome

Answer

Cri-du-chat syndrome

Question 7

Agarose gel electrophoresis from DNA of a population of cells as seen under ultraviolet light is shown below. What is the correct explanation for the finding seen in the band labeled as "C"?

Accepted Answer

Apoptotic cells

Answer

Predominantly necrotic cells

Answer

Mixed population of normal and apoptotic cells

Answer

A population of viable cells

Question 8

Identify the intracellular hyaline body.

Accepted Answer

Russell bodies

Answer

Corpora amylacea

Answer

Hyaline droplets

Answer

Mallory Hyaline bodies

Question 9

Ischemia-Reperfusion syndrome is characterized by:

Accepted Answer

Hypoxia and activation of inflammation

Answer

Thrombo embolic angiopathy

Answer

Build up of bicarbonate and Na+ ions

Answer

Acute mesenteric thrombosis

Question 10

Which of the following are metabolic causes of splenic enlargement?

Accepted Answer

Amyloid and Gaucher's disease

Answer

Porphyria and Felty's syndrome

Answer

Myelofibrosis and Weil's disease

Answer

Rickets and Still's disease

General Pathology — MCQs

General Pathology — MCQs

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