JAK-STAT pathway — Flashcards

Flashcard 1: Targets of JAK (tyrosine kinase associated-receptor pathway) include _____, which cause transcription of new mRNAs and new protein synthesis

Answer: STATs

Flashcard 2: NADPH oxidase

Answer: respiratory burst

Extra: O2O2-

Flashcard 3: Give the pathway by which glucagon/Epi regulate glycogen.

Answer: glucagon/Epi, adenylyl cyclase, cAMP, protein kinase A, glycogen phosphorylsae kinase, glycogen phosphorylase

Flashcard 4: Give the pathway by which insulin regulates glycogen.

Answer: insulin, tyrosine kinase receptor, protein phosphatases, inactivated glycogen phosphorylase kinase and glycogen phorphorylase

Flashcard 5: If an enyzme is a kinase, what do you know about its function?

Answer: uses ATP to add phosphate group to substrate

Flashcard 6: How does a direct enzyme-linked immunosorbent assay (ELISA) work?

Answer: 1. Patient's blood sample washed over a fixed antibody linked to a color-generating enzyme. 2. Any antibody-antibody-enzyme complex produces color change.

Flashcard 7: What is the function of cyclin proteins?

Answer: regulate cell cycle; concentrations are cyclic and phase-dependent, complex with and activate CDKs

Flashcard 8: When during the cell cycle are CDKs produced?

Answer: always; CDKs are constitutively expressed but inactive without the correct cyclin hence "cyclin-dependent"

Flashcard 9: Where does the exocytosis step of collagen synthesis occur?

Answer: plasma membrane of fibroblast

Flashcard 10: Which vesicular trafficking protein is involved in receptor-mediated endocytosis?

Answer: Clathrin (coated pits etc.)